ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEKICVAVRV RPPAPENGAS LWKVEDNRIS LHKSLDTPIT TASHAFDHVF DESSTNASVY 

        70         80         90        100        110        120 
ELLTKDIIHA AVEGFNGTAF AYGQTSSGKT FTMTGSETDP GIIRRSVRDV FERIHMISDR 

       130        140        150        160        170        180 
EFLIRVSYME IYNEEINDLL AVENQRLQIH EHLERGVFVA GLKEEIVSDA EQILKLIDSG 

       190        200        210        220        230        240 
EVNRHFGETN MNVHSSRSHT IFRMVIESRG KDNSSSDAIR VSVLNLVDLA GSERIAKTGA 

       250        260        270        280        290        300 
GGVRLQEGKY INKSLMILGN VINKLSDSTK LRAHIPYRDS KLTRILQPAL GGNAKTCIIC 

       310        320        330        340        350        360 
TIAPEEHHIE ESKGTLQFAS RAKRITNCAQ VNEILTDAAL LKRQKLEIEE LRMKLQGSHA 

       370        380        390        400        410        420 
EVLEQEILNL SNQMLKYELE CERLKTQLEE EKRKQKEQEN CIKEQQMKIE NLNNFVTNSD 

       430        440        450        460        470        480 
FKRNQSEDFI ISRKTPDGLC NVNDTSDVPG TPCFKSASRS FVVARSNNYS GLSDFSPMVH 

       490        500        510        520        530        540 
SLGDVADEDT WMKLNKGFVA DLDQIQFTPA VKCQPTPLSI ATTECPRENH SEVEDLKSRI 

       550        560        570        580        590        600 
QLLTNENDSL QVKFNEQVLL SNNLMQEMSE LKQETLTVKE IPNRLSESVA NCKDVYKDVI 

       610        620        630        640        650        660 
VTMKSLITDK ESPTANLLLG TTEITTSLLA TLETQFSMIM DGQKTGSSID HPLSDHWETL 

       670        680        690        700        710        720 
RVNLKNTTTL LLSDAQAKDE FLNSHNKGQE TAALEEKKLK SELIIIKERY NELEKELCLD 

       730        740        750        760        770        780 
KQLLEASRES HEKLIKEVQF LKEERDSLDR KISQSTQRLR VIASDKENAL KDLNVEVKRR 

       790        800        810        820 
KDMEEEIKHI SIAFATRHKS FVSFHSEIKS KMQKLTTQNS KAP 

References and documentation are available.

Number of amino acids: 823

Molecular weight: 93148.67

Theoretical pI: 5.85

Amino acid composition: 
Ala (A)  46	  5.6%
Arg (R)  44	  5.3%
Asn (N)  48	  5.8%
Asp (D)  44	  5.3%
Cys (C)  12	  1.5%
Gln (Q)  36	  4.4%
Glu (E)  82	 10.0%
Gly (G)  31	  3.8%
His (H)  23	  2.8%
Ile (I)  56	  6.8%
Leu (L)  84	 10.2%
Lys (K)  65	  7.9%
Met (M)  19	  2.3%
Phe (F)  26	  3.2%
Pro (P)  20	  2.4%
Ser (S)  74	  9.0%
Thr (T)  53	  6.4%
Trp (W)   3	  0.4%
Tyr (Y)  10	  1.2%
Val (V)  47	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 126
Total number of positively charged residues (Arg + Lys): 109

Atomic composition:

Carbon      C	      4047
Hydrogen    H	      6593
Nitrogen    N	      1153
Oxygen      O	      1297
Sulfur      S	        31

Formula: C4047H6593N1153O1297S31
Total number of atoms: 13121

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32150
Abs 0.1% (=1 g/l)   0.345, assuming all pairs of Cys residues form cystines


Ext. coefficient    31400
Abs 0.1% (=1 g/l)   0.337, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.10
This classifies the protein as unstable.



Aliphatic index: 88.49

Grand average of hydropathicity (GRAVY): -0.518