ProtParam
User-provided sequence:
10 20 30 40 50 60
MEKKVICQDI FWSCDGTSFV SVHNDFGIRQ YLVPEESNTD KLNRNLLLPF TRFFRNQSIV
70 80 90 100 110 120
SCAIDPFYTL YNENSDRLAG DRIVVGGKNF PLQLYSLMDG QCILSYDTMN KINGEYETVY
130 140 150 160 170 180
SVKIDVESRV YTGSCRNKVA IYDKSRRDAV WMNQSTKKAS KGRQSIISCF EEQPMGGQAL
190 200 210 220 230 240
SRGSLLCGSY ANEMFQVDCR HQRLERLNYT RTVAGGIVQI LTSDNGRYVY VVRRNSDAIS
250 260 270 280 290 300
IYDRRNLQHE LNVLRLPFRI HHNSAKLKAY IDTAYGLSMG TPQGTILNWG RDLVEFGGVP
310 320 330 340 350
SHNSVEDPLI TSIPPESEWR TNLDSTIPAT VVKNCPGDPE LFALSHGGTI SLCRFGG
References and
documentation are available.
Number of amino acids: 357
Molecular weight: 40307.63
Theoretical pI: 7.98
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1773
Hydrogen H 2778
Nitrogen N 504
Oxygen O 538
Sulfur S 17
Formula: C
1773H
2778N
504O
538S
17
Total number of atoms: 5610
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 46465
Abs 0.1% (=1 g/l) 1.153, assuming all pairs of Cys residues form cystines
Ext. coefficient 45840
Abs 0.1% (=1 g/l) 1.137, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.85
This classifies the protein as unstable.
Aliphatic index: 82.13
Grand average of hydropathicity (GRAVY): -0.358