ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEKNAEKKRI VLVPFPLQGH ITPMMQLGQA LNLKGFSITV ALGDSNRVSS TQHFPGFQFV 

        70         80         90        100        110        120 
TIPETIPLSQ HEALGVVEFV VTLNKTSETS FKDCIAHLLL QHGNDIACII YDELMYFSEA 

       130        140        150        160        170        180 
TAKDLRIPSV IFTTGSATNH VCSCILSKLN AEKFLIDMKD PEVQNMVVEN LHPLKYKDLP 

       190        200        210        220        230        240 
TSGMGPLERF LEICAEVVNK RTASAVIINT SSCLESSSLS WLKQELSIPV YPLGPLHITT 

       250        260        270        280        290        300 
SANFSLLEED RSCIEWLNKQ KLRSVIYISV GSIAHMETKE VLEMAWGLYN SNQPFLWVIR 

       310        320        330        340        350        360 
PGTESMPVEV SKIVSERGCI VKWAPQNEVL VHPAVGGFWS HCGWNSTLES IVEGVPMICR 

       370        380        390        400        410        420 
PFNGEQKLNA MYIESVWRVG VLLQGEVERG CVERAVKRLI VDDEGVGMRE RALVLKEKLN 

       430        440 
ASVRSGGSSY NALDELVHYL EAEYRNT 

References and documentation are available.

Number of amino acids: 447

Molecular weight: 49881.60

Theoretical pI: 5.71

Amino acid composition: 
Ala (A)  24	  5.4%
Arg (R)  18	  4.0%
Asn (N)  22	  4.9%
Asp (D)  12	  2.7%
Cys (C)  11	  2.5%
Gln (Q)  14	  3.1%
Glu (E)  39	  8.7%
Gly (G)  27	  6.0%
His (H)  12	  2.7%
Ile (I)  29	  6.5%
Leu (L)  47	 10.5%
Lys (K)  23	  5.1%
Met (M)  13	  2.9%
Phe (F)  15	  3.4%
Pro (P)  21	  4.7%
Ser (S)  39	  8.7%
Thr (T)  21	  4.7%
Trp (W)   8	  1.8%
Tyr (Y)  10	  2.2%
Val (V)  42	  9.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      2228
Hydrogen    H	      3536
Nitrogen    N	       592
Oxygen      O	       656
Sulfur      S	        24

Formula: C2228H3536N592O656S24
Total number of atoms: 7036

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59525
Abs 0.1% (=1 g/l)   1.193, assuming all pairs of Cys residues form cystines


Ext. coefficient    58900
Abs 0.1% (=1 g/l)   1.181, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.45
This classifies the protein as unstable.



Aliphatic index: 98.93

Grand average of hydropathicity (GRAVY): -0.003