ProtParam
User-provided sequence:
10 20 30 40 50 60
MEKNTAPFVV DTAPTTPPDK LVAPSIEEVN SNPNELRALR GQGRYFGVSD DDKDAIKEAA
70 80 90 100 110 120
PKCNNCSQRG HLKKDCPHII CSYCGATDDH YSRHCPKAIQ CSKCDEVGHY RSQCPHKWKK
130 140 150 160 170 180
VQCTLCKSKK HSKERCPSIW RAYILVDDNE KAKPKVLPFH TIYCYNCGGK GHFGDDCKEK
190 200 210 220 230 240
RSSRVPNEDG SAFTGSNLSV ELKQEYYRHM NRNSDENEDY QFSESIYDED PLPRPSHKRH
250 260 270 280 290 300
SQNDHSHSGR NKRRASNFHP PPYQKSNVIQ PTIRGETLSL NNNISKNSRY QNTKVNVSSI
310 320 330 340
SENMYGSRYN PSTYVDNNSI SNSSNYRNYN SYQPYRSGTL GKRR
References and
documentation are available.
Number of amino acids: 344
Molecular weight: 39343.55
Theoretical pI: 9.06
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 53
Atomic composition:
Carbon C 1685
Hydrogen H 2616
Nitrogen N 520
Oxygen O 538
Sulfur S 18
Formula: C
1685H
2616N
520O
538S
18
Total number of atoms: 5377
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41675
Abs 0.1% (=1 g/l) 1.059, assuming all pairs of Cys residues form cystines
Ext. coefficient 40800
Abs 0.1% (=1 g/l) 1.037, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.51
This classifies the protein as unstable.
Aliphatic index: 47.03
Grand average of hydropathicity (GRAVY): -1.181