ProtParam
User-provided sequence:
10 20 30 40 50 60
MEKPNPRRPS NSVLPYETPR LRDHYLLGKK LGQGQFGTTY LCTEKSSSAN YACKSIPKRK
70 80 90 100 110 120
LVCREDYEDV WREIQIMHHL SEHPNVVRIK GTYEDSVFVH IVMEVCEGGE LFDRIVSKGC
130 140 150 160 170 180
FSEREAAKLI KTILGVVEAC HSLGVMHRDL KPENFLFDSP SDDAKLKATD FGLSVFYKPG
190 200 210 220 230 240
QYLYDVVGSP YYVAPEVLKK CYGPEIDVWS AGVILYILLS GVPPFWAETE SGIFRQILQG
250 260 270 280 290 300
KIDFKSDPWP TISEGAKDLI YKMLDRSPKK RISAHEALCH PWIVDEHAAP DKPLDPAVLS
310 320 330 340 350 360
RLKQFSQMNK IKKMALRVIA ERLSEEEIGG LKELFKMIDT DNSGTITFEE LKAGLKRVGS
370 380 390 400 410 420
ELMESEIKSL MDAADIDNSG TIDYGEFLAA TLHINKMERE ENLVVAFSYF DKDGSGYITI
430 440 450 460 470 480
DELQQACTEF GLCDTPLDDM IKEIDLDNDG KIDFSEFTAM MKKGDGVGRS RTMRNNLNFN
490 500
IAEAFGVEDT SSTAKSDDSP K
References and
documentation are available.
Number of amino acids: 501
Molecular weight: 56416.37
Theoretical pI: 5.22
Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 63
Atomic composition:
Carbon C 2511
Hydrogen H 3940
Nitrogen N 660
Oxygen O 765
Sulfur S 25
Formula: C
2511H
3940N
660O
765S
25
Total number of atoms: 7901
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 53455
Abs 0.1% (=1 g/l) 0.948, assuming all pairs of Cys residues form cystines
Ext. coefficient 52830
Abs 0.1% (=1 g/l) 0.936, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.96
This classifies the protein as unstable.
Aliphatic index: 82.51
Grand average of hydropathicity (GRAVY): -0.367