ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEKYEKLEKV GEGTYGKVYK AMEKGTGKLV ALKKTRLEMD EEGIPPTALR EISLLQMLST 

        70         80         90        100        110        120 
SIYVVRLLCV EHVHQPSTKS QSTKSNLYLV FEYLDTDLKK FIDSYRKGPN PKPLEPFLIQ 

       130        140        150        160        170        180 
KLMFQLCKGV AHCHSHGVLH RDLKPQNLLL VKDKELLKIA DLGLGRAFTV PLKSYTHEIV 

       190        200        210        220        230        240 
TLWYRAPEVL LGSTHYSTGV DMWSVGCIFA EMVRRQALFP GDSEFQQLLH IFRLLGTPTE 

       250        260        270        280        290        300 
QQWPGVSTLR DWHVYPKWEP QDLTLAVPSL SPQGVDLLTK MLKYNPAERI SAKTALDHPY 


FDSLDKSQF 

References and documentation are available.

Number of amino acids: 309

Molecular weight: 35318.08

Theoretical pI: 8.67

Amino acid composition: 
Ala (A)  13	  4.2%
Arg (R)  12	  3.9%
Asn (N)   4	  1.3%
Asp (D)  15	  4.9%
Cys (C)   4	  1.3%
Gln (Q)  14	  4.5%
Glu (E)  20	  6.5%
Gly (G)  18	  5.8%
His (H)  11	  3.6%
Ile (I)  10	  3.2%
Leu (L)  45	 14.6%
Lys (K)  27	  8.7%
Met (M)   8	  2.6%
Phe (F)  11	  3.6%
Pro (P)  19	  6.1%
Ser (S)  20	  6.5%
Thr (T)  19	  6.1%
Trp (W)   5	  1.6%
Tyr (Y)  13	  4.2%
Val (V)  21	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      1610
Hydrogen    H	      2535
Nitrogen    N	       417
Oxygen      O	       450
Sulfur      S	        12

Formula: C1610H2535N417O450S12
Total number of atoms: 5024

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47120
Abs 0.1% (=1 g/l)   1.334, assuming all pairs of Cys residues form cystines


Ext. coefficient    46870
Abs 0.1% (=1 g/l)   1.327, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.33
This classifies the protein as stable.



Aliphatic index: 93.33

Grand average of hydropathicity (GRAVY): -0.274