ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MELEPTLFGI IEALAPQLLS QSHLQTFVSD VVNLLRSSTK SATQLGPLID FYKLQSLDSP 

        70         80         90        100        110        120 
ETTIMWHKIE KFLDALFGIQ NTDDMVKYLS VFQSLLPSNY RAKIVQKSSG LNMENLANHE 

       130        140        150        160        170        180 
HLLSPVRAPS IYTEASFENM DRFSERRSMV SSPNRYVPSS TYSSVTLRQL SNPYYVNTIP 

       190        200        210        220        230        240 
EEDILKYVSY TLLATTSALF PFDHEQIQIP SKIPNFESGL LHLIFEAGLL YQSLGYKVEK 

       250        260        270        280        290        300 
FRMLNISPMK KALIIEISEE LQNYTAFVNN LVSSGTVVSL KSLYREIYEN IIRLRIYCRF 

       310        320        330        340        350        360 
TEHLEELSGD TFLIELNIFK SHGDLTIRKI ATNLFNSMIS LYYEYLMNWL TKGLLRATYG 

       370        380        390        400        410        420 
EFFIAENTDT NGTDDDFIYH IPIEFNQERV PAFIPKELAY KIFMIGKSYI FLEKYCKEVQ 

       430        440        450        460        470        480 
WTNEFSKKYH VLYQSNSYRG ISTNFFEIIN DQYSEIVNHT NQILNQKFHY RDVVFALKNI 

       490        500        510        520        530        540 
LLMGKSDFMD ALIEKANDIL ATPSDSLPNY KLTRVLQEAV QLSSLRHLMN SPRNSSVING 

       550        560        570        580        590        600 
LDARVLDLGH GSVGWDVFTL DYILYPPLSL VLNVNRPFGR KEYLRIFNFL WRFKKNNYFY 

       610        620        630        640        650        660 
QKEMLKSNDI IRSFKKIRGY NPLIRDIINK LSRISILRTQ FQQFNSKMES YYLNCIIEEN 

       670        680        690        700        710        720 
FKEMTRKLQR TENKSQNQFD LIRLNNGTIE LNGILTPKAE VLTKSSSSKP QKHAIEKTLN 

       730        740        750        760        770        780 
IDELESVHNT FLTNILSHKL FATNTSEISV GDYSGQPYPT SLVLLLNSVY EFVKVYCNLN 

       790        800        810        820        830        840 
DIGYEIFIKM NLNDHEASNG LLGKFNTNLK EIVSQYKNFK DRLYIFRADL KNDGDEELFL 


LSKSLR 

References and documentation are available.

Number of amino acids: 846

Molecular weight: 98226.60

Theoretical pI: 7.03

Amino acid composition: 
Ala (A)  29	  3.4%
Arg (R)  37	  4.4%
Asn (N)  66	  7.8%
Asp (D)  36	  4.3%
Cys (C)   4	  0.5%
Gln (Q)  31	  3.7%
Glu (E)  57	  6.7%
Gly (G)  30	  3.5%
His (H)  18	  2.1%
Ile (I)  68	  8.0%
Leu (L) 107	 12.6%
Lys (K)  55	  6.5%
Met (M)  18	  2.1%
Phe (F)  50	  5.9%
Pro (P)  29	  3.4%
Ser (S)  78	  9.2%
Thr (T)  44	  5.2%
Trp (W)   5	  0.6%
Tyr (Y)  45	  5.3%
Val (V)  39	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 93
Total number of positively charged residues (Arg + Lys): 92

Atomic composition:

Carbon      C	      4467
Hydrogen    H	      6954
Nitrogen    N	      1150
Oxygen      O	      1297
Sulfur      S	        22

Formula: C4467H6954N1150O1297S22
Total number of atoms: 13890

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    94800
Abs 0.1% (=1 g/l)   0.965, assuming all pairs of Cys residues form cystines


Ext. coefficient    94550
Abs 0.1% (=1 g/l)   0.963, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.03
This classifies the protein as unstable.



Aliphatic index: 97.47

Grand average of hydropathicity (GRAVY): -0.243