ProtParam
User-provided sequence:
10 20 30 40 50 60
MELILNSLIS DDLTEEQKRL SLDFLQDILQ SNTKDYESYF SSRAVPGSIT EDIAEIDAEL
70 80 90 100 110 120
SALDRKIRKT LLDNTSQIIG NILENDDRAQ LDDIAKSLEQ LWELDTNINK AADRNVTNDD
130 140 150 160 170 180
INNESVSIDD FLEDDKEDND TGRIMTTESN NLARKKKEDE FHKALSRLRN RISTKEDDKD
190 200 210 220 230 240
DIRSDTLVTV LENLDSITDL MELPFLARTC IRTGHYQEAV MLYTHTTSLR SRFPGSTIVD
250 260 270 280 290 300
EVCEKVLNEI STTMLSGLVK LLSTNVSVNS LKKILQYLNS IPPFDGKTNK SLLSVFLAMR
310 320 330 340 350 360
YKFITDEIAS YPLDVESSNE SLIEMMVKRK IEVLREHVYM SLNVFLKSFL YDTNDLEIPF
370 380 390 400 410 420
PEELESTVLR INGTNEEKEI EEKEKETKKE EYQKQDSVAN NEEDVTENKS IEDVQEEVQG
430 440 450 460 470 480
KVEGEDDGAE RKTENEIENE TVNKTEDKAE KEKEEEVNTK DNKAEKEEEE INKVEVTPEE
490 500 510 520 530 540
PSKSIDNKAE KEEEEINKVE VTPEEPSKKI RTSKRENKIP TNAVMLQFVD KCITYVLKDL
550 560 570 580 590 600
TRGLNSIKLS DSVCLQLVYC SFRLCDLNRN YHHLFLKKIN DTSLFTTEQL ARAIDKRAEL
ASKYIYS
References and
documentation are available.
Number of amino acids: 607
Molecular weight: 69829.07
Theoretical pI: 4.63
Total number of negatively charged residues (Asp + Glu): 131
Total number of positively charged residues (Arg + Lys): 83
Atomic composition:
Carbon C 3028
Hydrogen H 4905
Nitrogen N 817
Oxygen O 1035
Sulfur S 16
Formula: C
3028H
4905N
817O
1035S
16
Total number of atoms: 9801
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28225
Abs 0.1% (=1 g/l) 0.404, assuming all pairs of Cys residues form cystines
Ext. coefficient 27850
Abs 0.1% (=1 g/l) 0.399, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.44
This classifies the protein as unstable.
Aliphatic index: 88.14
Grand average of hydropathicity (GRAVY): -0.723