ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MELLRGLGVL FLLHVCGSNR IPESGGDNGV FDIFELIGGA RKVPGRRLVK GQDLSSPAFR 

        70         80         90        100        110        120 
IENANLIPPV PDDKFQDLLD AVWADKGFIF LASLRQMKKT RGTLLAVERK DNSGQIFSVV 

       130        140        150        160        170        180 
SNGKAGTLDL SLSLPGKQQV VSVEEALLAT GQWKSITLFV QEDRAQLYID CDKMESAELD 

       190        200        210        220        230        240 
VPIQSIFTRD LANVARLRVA KGDVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTNVLL 

       250        260        270        280        290        300 
TLDNNVVNGS SPAIRTNYIG HKTKDLQAIC GLSCDELSSM VLELRGLRTI VTTLQDSIRK 

       310        320        330        340        350        360 
VTEENRELAS ELRRPPLCFH NGVQYRNNEE WTVDSCTECH CQNSVTICKK VSCPIMPCSN 

       370        380        390        400        410        420 
ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSATCGNGIQ QRGRSCDSLN NRCEGSSVQT 

       430        440        450        460        470        480 
RTCHIQECDK RFKQDGGWSH WSPWSSCSVT CGDGVITRIR LCNSPSPQMN GKPCEGEARE 

       490        500        510        520        530        540 
TKACKKDACP INGGWSPWSL WDICSVTCGG GVQRRSRLCN NPTPQFGGKD CVGDVTENQV 

       550        560        570        580        590        600 
CNKQDCPIDG CLSNPCFAGA KCTSYPDGSW KCGACPPGYS GNGIQCKDVD ECKEVPDACF 

       610        620        630        640        650        660 
NHNGEHRCKN TDPGYNCLPC PPRFTGSQPF GRGVEHAMAN KQVCKPRNPC TDGTHDCNKN 

       670        680        690        700        710        720 
AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN ENLVCVANAT YHCKKDNCPN 

       730        740        750        760        770        780 
LPNSGQEDYD KDGIGDACDD DDDNDKIPDD RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN 

       790        800        810        820        830        840 
HNPDQADTDN NGEGDACAVD IDGDGILNER DNCQYVYNVD QRDTDMDGVG DQCDNCPLEH 

       850        860        870        880        890        900 
NPDQLDSDSD RIGDTCDNNQ AIDEDGHQNN LENCPYVPNA NQADHDKDGK GDACDHDDDN 

       910        920        930        940        950        960 
DGIPDDRDNC RLVPNPDQKD SDGDGRGDAC KDDFDHDNVP DIDDICPENV DISETDFRRF 

       970        980        990       1000       1010       1020 
QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY DEFNAVDFSG TFFINTERDD 

      1030       1040       1050       1060       1070       1080 
DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL 

      1090       1100       1110       1120       1130       1140 
WHTGNTPGQV RTLWHDPRHI GWKDFTAYRW RLSHRPKTGL IRVVMYEGKK IMADSGPIYD 

      1150       1160       1170 
KTYAGGRLGL FVFSQEMVFF SDMKYECRDS 

References and documentation are available.

Number of amino acids: 1170

Molecular weight: 129671.26

Theoretical pI: 4.74

Amino acid composition: 
Ala (A)  52	  4.4%
Arg (R)  64	  5.5%
Asn (N)  84	  7.2%
Asp (D) 131	 11.2%
Cys (C)  70	  6.0%
Gln (Q)  50	  4.3%
Glu (E)  50	  4.3%
Gly (G) 103	  8.8%
His (H)  25	  2.1%
Ile (I)  45	  3.8%
Leu (L)  68	  5.8%
Lys (K)  54	  4.6%
Met (M)  15	  1.3%
Phe (F)  36	  3.1%
Pro (P)  68	  5.8%
Ser (S)  74	  6.3%
Thr (T)  56	  4.8%
Trp (W)  22	  1.9%
Tyr (Y)  30	  2.6%
Val (V)  73	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 181
Total number of positively charged residues (Arg + Lys): 118

Atomic composition:

Carbon      C	      5530
Hydrogen    H	      8508
Nitrogen    N	      1622
Oxygen      O	      1827
Sulfur      S	        85

Formula: C5530H8508N1622O1827S85
Total number of atoms: 17572

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   170075
Abs 0.1% (=1 g/l)   1.312, assuming all pairs of Cys residues form cystines


Ext. coefficient   165700
Abs 0.1% (=1 g/l)   1.278, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.92
This classifies the protein as unstable.



Aliphatic index: 60.21

Grand average of hydropathicity (GRAVY): -0.703