ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MELPKLLISL FLFSFSSFFL GAESDYPQHS NLKRLRPNRL FVFGDSYADT GNIRKSLSDS 

        70         80         90        100        110        120 
WKIPYGITFP QKPSGRFSDG RVATDFLARY LGIKSPIPYT WKDYAGKERL LYGMNYAYGG 

       130        140        150        160        170        180 
TGVFKTKDNP LPNMTTQIDY FQRVLAAGNI YSPSDLPSSL ALVSVAGNDY ATFLALKRPL 

       190        200        210        220        230        240 
TELPAFMKQV VDQIAVNAMR IHKLGVNKIV IPSMQPLGCL PSITVFNSFQ RCNATDNAST 

       250        260        270        280        290        300 
NLHNYLLHKA IARLNNETKP STFVVLDHYN AFLTVFKNKG PEPGVSRFGN PLKPCCVGVN 

       310        320        330        340        350 
SSYDCSNVDE KGEKKYIICE DPKAAFFWDI FHPSEEGWRS VYSVLHKHLK AIWI 

References and documentation are available.

Number of amino acids: 354

Molecular weight: 39828.79

Theoretical pI: 9.24

Amino acid composition: 
Ala (A)  23	  6.5%
Arg (R)  15	  4.2%
Asn (N)  22	  6.2%
Asp (D)  18	  5.1%
Cys (C)   6	  1.7%
Gln (Q)   8	  2.3%
Glu (E)  11	  3.1%
Gly (G)  22	  6.2%
His (H)   8	  2.3%
Ile (I)  19	  5.4%
Leu (L)  35	  9.9%
Lys (K)  25	  7.1%
Met (M)   6	  1.7%
Phe (F)  23	  6.5%
Pro (P)  24	  6.8%
Ser (S)  30	  8.5%
Thr (T)  16	  4.5%
Trp (W)   5	  1.4%
Tyr (Y)  17	  4.8%
Val (V)  21	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1822
Hydrogen    H	      2789
Nitrogen    N	       475
Oxygen      O	       506
Sulfur      S	        12

Formula: C1822H2789N475O506S12
Total number of atoms: 5604

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53205
Abs 0.1% (=1 g/l)   1.336, assuming all pairs of Cys residues form cystines


Ext. coefficient    52830
Abs 0.1% (=1 g/l)   1.326, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.28
This classifies the protein as stable.



Aliphatic index: 83.19

Grand average of hydropathicity (GRAVY): -0.190