ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MELRVGNKYR LGRKIGSGSF GDIYLGTTIN TGEEVAIKLE CIRTKHPQLH IESKFYKTMQ 

        70         80         90        100        110        120 
GGIGIPRIIW CGSEGDYNVM VMELLGPSLE DLFNFCSRRF SLKTVLLLAD QMISRIDYIH 

       130        140        150        160        170        180 
SRDFIHRDIK PDNFLMGLGK KGNLVYIIDF GLAKKFRDAR SLKHIPYREN KNLTGTARYA 

       190        200        210        220        230        240 
SINTHLGIEQ SRRDDLESLG YVLMYFNLGA LPWQGLKAAN KRQKYERISE KKLSTSIVVL 

       250        260        270        280        290        300 
CKGFPSEFVN YLNFCRQMHF DQRPDYCHLR KLFRNLFHRL GFTYDYVFDW NLLKFGGPRN 

       310        320        330        340        350        360 
PQAIQQAQDG ADGQAGHDAV AAAAAVAAAA AASSHQQQQH KVNAALGGGG GSAAQQQLQG 

       370        380        390        400        410        420 
GQTLAMLGGN GGGNGSQLIG GNGLNMDDSM AATNSSRPPY DTPERRPSIR MRQGGGGGGG 

       430        440 
GVGVGGMPSG GGGGGVGNAK 

References and documentation are available.

Number of amino acids: 440

Molecular weight: 47957.58

Theoretical pI: 9.54

Amino acid composition: 
Ala (A)  33	  7.5%
Arg (R)  29	  6.6%
Asn (N)  23	  5.2%
Asp (D)  22	  5.0%
Cys (C)   6	  1.4%
Gln (Q)  24	  5.5%
Glu (E)  15	  3.4%
Gly (G)  63	 14.3%
His (H)  12	  2.7%
Ile (I)  25	  5.7%
Leu (L)  41	  9.3%
Lys (K)  24	  5.5%
Met (M)  13	  3.0%
Phe (F)  19	  4.3%
Pro (P)  15	  3.4%
Ser (S)  26	  5.9%
Thr (T)  14	  3.2%
Trp (W)   3	  0.7%
Tyr (Y)  16	  3.6%
Val (V)  17	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2111
Hydrogen    H	      3325
Nitrogen    N	       625
Oxygen      O	       618
Sulfur      S	        19

Formula: C2111H3325N625O618S19
Total number of atoms: 6698

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40715
Abs 0.1% (=1 g/l)   0.849, assuming all pairs of Cys residues form cystines


Ext. coefficient    40340
Abs 0.1% (=1 g/l)   0.841, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.24
This classifies the protein as unstable.



Aliphatic index: 77.20

Grand average of hydropathicity (GRAVY): -0.381