ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MELSPTYQTE RFTKFSDTLK EFKIEQNNEQ NPIDPFNIIR EFRSAAGQLA LDLANSGDES 

        70         80         90        100        110        120 
NVISSKDWEL EARFWHLVEL LLVFRNADLD LDEMELHPYN SRGLFEKKLM QDNKQLYQIW 

       130        140        150        160        170        180 
IVMVWLKENT YVMERPKNVP TSKWLNSITS GGLKSCDLDF PLRENTNVLD VKDKEEDHIF 

       190        200        210        220        230        240 
FKYIYELILA GAIDEALEEA KLSDNISICM ILCGIQEYLN PVIDTQIANE FNTQQGIKKH 

       250        260        270        280        290        300 
SLWRRTVYSL SQQAGLDPYE RAIYSYLSGA IPNQEVLQYS DWESDLHIHL NQILQTEIEN 

       310        320        330        340        350        360 
YLLENNQVGT DELILPLPSH ALTVQEVLNR VASRHPSESE HPIRVLMASV ILDSLPSVIH 

       370        380        390        400        410        420 
SSVEMLLDVV KGTEASNDII DKPYLLRIVT HLAICLDIIN PGSVEEVDKS KLITTYISLL 

       430        440        450        460        470        480 
KLQGLYENIP IYATFLNESD CLEACSFILS SLEDPQVRKK QIETINFLRL PASNILRRTT 

       490        500        510        520        530        540 
QRVFDETEQE YSPSNEISIS FDVNNIDMHL IYGVEWLIEG KLYVDAVHSI IALSRRFLLN 

       550        560        570        580        590        600 
GRVKALEQFM ERNNIGEICK NYELEKIADN ISKDENEDQF LEEITQYEHL IKGIREYEEW 

       610        620        630        640        650        660 
QKSVSLLSSE SNIPTLIEKL QGFSKDTFEL IKTFLVDLTS SNFADSADYE ILYEIRALYT 

       670        680        690        700        710        720 
PFLLMELHKK LVEAAKLLKI PKFISEALAF TSLVANENDK IYLLFQSSGK LKEYLDLVAR 


TATLSN 

References and documentation are available.

Number of amino acids: 726

Molecular weight: 83635.27

Theoretical pI: 4.75

Amino acid composition: 
Ala (A)  36	  5.0%
Arg (R)  27	  3.7%
Asn (N)  44	  6.1%
Asp (D)  42	  5.8%
Cys (C)   7	  1.0%
Gln (Q)  30	  4.1%
Glu (E)  72	  9.9%
Gly (G)  21	  2.9%
His (H)  15	  2.1%
Ile (I)  64	  8.8%
Leu (L)  97	 13.4%
Lys (K)  42	  5.8%
Met (M)  11	  1.5%
Phe (F)  28	  3.9%
Pro (P)  24	  3.3%
Ser (S)  60	  8.3%
Thr (T)  32	  4.4%
Trp (W)   9	  1.2%
Tyr (Y)  28	  3.9%
Val (V)  37	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 114
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      3766
Hydrogen    H	      5920
Nitrogen    N	       962
Oxygen      O	      1149
Sulfur      S	        18

Formula: C3766H5920N962O1149S18
Total number of atoms: 11815

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    91595
Abs 0.1% (=1 g/l)   1.095, assuming all pairs of Cys residues form cystines


Ext. coefficient    91220
Abs 0.1% (=1 g/l)   1.091, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.99
This classifies the protein as unstable.



Aliphatic index: 106.23

Grand average of hydropathicity (GRAVY): -0.220