ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MELSSASAIL SHSSSAAQLL RPKLGFIDLL PRRAMIVSSP SSSLPRFLRM ESQSQLRQSI 

        70         80         90        100        110        120 
SCSASSSSSM ALGRIGEVKR VTKETNVSVK INLDGTGVAD SSSGIPFLDH MLDQLASHGL 

       130        140        150        160        170        180 
FDVHVRATGD VHIDDHHTNE DIALAIGTAL LKALGERKGI NRFGDFTAPL DEALIHVSLD 

       190        200        210        220        230        240 
LSGRPYLGYN LEIPTQRVGT YDTQLVEHFF QSLVNTSGMT LHIRQLAGEN SHHIIEATFK 

       250        260        270 
AFARALRQAT ETDPRRGGTI PSSKGVLSRS 

References and documentation are available.

Number of amino acids: 270

Molecular weight: 29225.10

Theoretical pI: 7.23

Amino acid composition: 
Ala (A)  22	  8.1%
Arg (R)  19	  7.0%
Asn (N)   7	  2.6%
Asp (D)  15	  5.6%
Cys (C)   1	  0.4%
Gln (Q)  10	  3.7%
Glu (E)  12	  4.4%
Gly (G)  20	  7.4%
His (H)  12	  4.4%
Ile (I)  17	  6.3%
Leu (L)  32	 11.9%
Lys (K)   8	  3.0%
Met (M)   6	  2.2%
Phe (F)  10	  3.7%
Pro (P)  10	  3.7%
Ser (S)  36	 13.3%
Thr (T)  16	  5.9%
Trp (W)   0	  0.0%
Tyr (Y)   3	  1.1%
Val (V)  14	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1274
Hydrogen    H	      2064
Nitrogen    N	       376
Oxygen      O	       397
Sulfur      S	         7

Formula: C1274H2064N376O397S7
Total number of atoms: 4118

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     4470
Abs 0.1% (=1 g/l)   0.153, assuming all pairs of Cys residues form cystines


Ext. coefficient     4470
Abs 0.1% (=1 g/l)   0.153, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.83
This classifies the protein as unstable.



Aliphatic index: 93.96

Grand average of hydropathicity (GRAVY): -0.143