ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MELTLNSSSS LIKRKDAKSS RNQESSSNNM TFAKMKPPTY QFQAKNSVKE MKFTHEKTFT 

        70         80         90        100        110        120 
PEGETLEKWE KLHVLSYPHS KNDASVPVFV MLPLDTVTMS GHLNKPRAMN ASLMALKGAG 

       130        140        150        160        170        180 
VEGVMVDAWW GLVEKDGPMN YNWEGYAELI QMVQKHGLKL QVVMSFHQCG GNVGDSCSIP 

       190        200        210        220        230        240 
LPPWVLEEIS KNPDLVYTDK SGRRNPEYIS LGCDSVPVLR GRTPIQVYSD FMRSFRERFE 

       250        260        270        280        290        300 
GYIGGVIAEI QVGMGPCGEL RYPSYPESNG TWRFPGIGEF QCYDKYMKSS LQAYAESIGK 

       310        320        330        340        350        360 
TNWGTSGPHD AGEYKNLPED TEFFRRDGTW NSEYGKFFME WYSGKLLEHG DQLLSSAKGI 

       370        380        390        400        410        420 
FQGSGAKLSG KVAGIHWHYN TRSHAAELTA GYYNTRNHDG YLPIAKMFNK HGVVLNFTCM 

       430        440        450        460        470        480 
EMKDGEQPEH ANCSPEGLVK QVQNATRQAG TELAGENALE RYDSSAFGQV VATNRSDSGN 

       490        500        510        520        530        540 
GLTAFTYLRM NKRLFEGQNW QQLVEFVKNM KEGGHGRRLS KEDTTGSDLY VGFVKGKIAE 


NVEEAALV 

References and documentation are available.

Number of amino acids: 548

Molecular weight: 61353.16

Theoretical pI: 6.59

Amino acid composition: 
Ala (A)  32	  5.8%
Arg (R)  23	  4.2%
Asn (N)  31	  5.7%
Asp (D)  21	  3.8%
Cys (C)   7	  1.3%
Gln (Q)  21	  3.8%
Glu (E)  43	  7.8%
Gly (G)  54	  9.9%
His (H)  15	  2.7%
Ile (I)  15	  2.7%
Leu (L)  41	  7.5%
Lys (K)  38	  6.9%
Met (M)  21	  3.8%
Phe (F)  23	  4.2%
Pro (P)  24	  4.4%
Ser (S)  44	  8.0%
Thr (T)  27	  4.9%
Trp (W)  11	  2.0%
Tyr (Y)  23	  4.2%
Val (V)  34	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2715
Hydrogen    H	      4168
Nitrogen    N	       748
Oxygen      O	       823
Sulfur      S	        28

Formula: C2715H4168N748O823S28
Total number of atoms: 8482

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    95145
Abs 0.1% (=1 g/l)   1.551, assuming all pairs of Cys residues form cystines


Ext. coefficient    94770
Abs 0.1% (=1 g/l)   1.545, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.93
This classifies the protein as stable.



Aliphatic index: 63.69

Grand average of hydropathicity (GRAVY): -0.573