ProtParam
User-provided sequence:
10 20 30 40 50 60
MELWTEARAL KASLRGESTI SLKHHQVIVS EDLSRTSSLP EDFSVECFLD FSEGQKEEEE
70 80 90 100 110 120
EVVSVSSSQE QEEQEHDCVF SSQPCIFDQL PSLPDEDVEE LEWVSRVVDD CSSPEVSLLL
130 140 150 160 170 180
TQTHKTKPSF SRIPVKPRTK RSRNSLTGSR VWPLVSTNHQ HAATEQLRKK KQETVLVFQR
190 200 210 220 230 240
RCSHCGTNNT PQWRTGPVGP KTLCNACGVR FKSGRLCPEY RPADSPTFSN EIHSNLHRKV
250 260
LELRKSKELG EETGEASTKS DPVKFGSKW
References and
documentation are available.
Number of amino acids: 269
Molecular weight: 30468.05
Theoretical pI: 6.03
Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1319
Hydrogen H 2094
Nitrogen N 384
Oxygen O 426
Sulfur S 10
Formula: C
1319H
2094N
384O
426S
10
Total number of atoms: 4233
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29490
Abs 0.1% (=1 g/l) 0.968, assuming all pairs of Cys residues form cystines
Ext. coefficient 28990
Abs 0.1% (=1 g/l) 0.951, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 56.26
This classifies the protein as unstable.
Aliphatic index: 66.21
Grand average of hydropathicity (GRAVY): -0.762