ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEMIRGRGIP NGVVQVPNLS ANNLLQKFFI ASNHFCQRDS EHGENQQEAK KREDEAEEDE 

        70         80         90        100        110        120 
KDVELTLGLS LNGQFGTDPR SRKRRHFELG RSSSIPEGFV FDEQRSGGGN GGDMRRIVGR 

       130        140        150        160        170        180 
GGSDMFQLDR TRSLPVVTEM DIEKERKVSE KTRAFMESPV TNRGAYLTKD KNRGQAVETE 

       190        200        210        220        230        240 
KPRAFLEFKI PPTKEGKKEK DRLVVTGPVN GKGKNGNTAK KQKNNVENSG MEKARNILED 

       250        260        270        280        290        300 
MPCVSTRDVG ADGKRVEGFL YWYGGNKEEV KIVCVCHGSF LSPAEFVRHG GGTVSDDDGG 

       310 
DVMINPLRHI VVKLPSSSI 

References and documentation are available.

Number of amino acids: 319

Molecular weight: 35454.88

Theoretical pI: 8.55

Amino acid composition: 
Ala (A)  12	  3.8%
Arg (R)  26	  8.2%
Asn (N)  19	  6.0%
Asp (D)  19	  6.0%
Cys (C)   4	  1.3%
Gln (Q)  10	  3.1%
Glu (E)  30	  9.4%
Gly (G)  36	 11.3%
His (H)   6	  1.9%
Ile (I)  12	  3.8%
Leu (L)  18	  5.6%
Lys (K)  26	  8.2%
Met (M)   9	  2.8%
Phe (F)  14	  4.4%
Pro (P)  14	  4.4%
Ser (S)  21	  6.6%
Thr (T)  13	  4.1%
Trp (W)   1	  0.3%
Tyr (Y)   3	  0.9%
Val (V)  26	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      1524
Hydrogen    H	      2457
Nitrogen    N	       465
Oxygen      O	       484
Sulfur      S	        13

Formula: C1524H2457N465O484S13
Total number of atoms: 4943

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    10220
Abs 0.1% (=1 g/l)   0.288, assuming all pairs of Cys residues form cystines


Ext. coefficient     9970
Abs 0.1% (=1 g/l)   0.281, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.45
This classifies the protein as unstable.



Aliphatic index: 64.08

Grand average of hydropathicity (GRAVY): -0.811