ProtParam
User-provided sequence:
10 20 30 40 50 60
MEMSLRPLLS VFVLGLVSTP STLAQDDPRY TKFLTQHYDA KPKGRDARYC ESMMRRRGLT
70 80 90 100 110 120
SPCKEVNTFI HGNKGSIKAI CGANGSPYGE NLRISQSPFQ ITTCKHTGGS PRPPCRYRAS
130 140
AGFRHVVIAC ENGLPVHFDE SFISL
References and
documentation are available.
Number of amino acids: 145
Molecular weight: 16079.46
Theoretical pI: 9.44
Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 19
Atomic composition:
Carbon C 704
Hydrogen H 1115
Nitrogen N 207
Oxygen O 205
Sulfur S 10
Formula: C
704H
1115N
207O
205S
10
Total number of atoms: 2241
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7825
Abs 0.1% (=1 g/l) 0.487, assuming all pairs of Cys residues form cystines
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.463, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.38
This classifies the protein as unstable.
Aliphatic index: 67.93
Grand average of hydropathicity (GRAVY): -0.375