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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEMSLRPLLS VFVLGLVSTP STLAQDDPRY TKFLTQHYDA KPKGRDARYC ESMMRRRGLT

70 80 90 100 110 120
SPCKEVNTFI HGNKGSIKAI CGANGSPYGE NLRISQSPFQ ITTCKHTGGS PRPPCRYRAS

130 140
AGFRHVVIAC ENGLPVHFDE SFISL


References and documentation are available.

Number of amino acids: 145 Molecular weight: 16079.46 Theoretical pI: 9.44
Amino acid composition: 
Ala (A) 8 5.5% Arg (R) 12 8.3% Asn (N) 5 3.4% Asp (D) 5 3.4% Cys (C) 6 4.1% Gln (Q) 4 2.8% Glu (E) 6 4.1% Gly (G) 12 8.3% His (H) 5 3.4% Ile (I) 7 4.8% Leu (L) 11 7.6% Lys (K) 7 4.8% Met (M) 4 2.8% Phe (F) 7 4.8% Pro (P) 11 7.6% Ser (S) 14 9.7% Thr (T) 9 6.2% Trp (W) 0 0.0% Tyr (Y) 5 3.4% Val (V) 7 4.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 11 Total number of positively charged residues (Arg + Lys): 19 Atomic composition: Carbon C 704 Hydrogen H 1115 Nitrogen N 207 Oxygen O 205 Sulfur S 10 Formula: C704H1115N207O205S10 Total number of atoms: 2241 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 7825 Abs 0.1% (=1 g/l) 0.487, assuming all pairs of Cys residues form cystines Ext. coefficient 7450 Abs 0.1% (=1 g/l) 0.463, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 50.38 This classifies the protein as unstable. Aliphatic index: 67.93 Grand average of hydropathicity (GRAVY): -0.375