ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEMTDFELTS NSQSNLAIPT NFKSTLPPRK RAKTKEEKEQ RRIERILRNR RAAHQSREKK 

        70         80         90        100        110        120 
RLHLQYLERK CSLLENLLNS VNLEKLADHE DALTCSHDAF VASLDEYRDF QSTRGASLDT 

       130        140        150        160        170        180 
RASSHSSSDT FTPSPLNCTM EPATLSPKSM RDSASDQETS WELQMFKTEN VPESTTLPAV 

       190        200        210        220        230 
DNNNLFDAVA SPLADPLCDD IAGNSLPFDN SIDLDNWRNP EAQSGLNSFE LNDFFITS 

References and documentation are available.

Number of amino acids: 238

Molecular weight: 26904.74

Theoretical pI: 4.97

Amino acid composition: 
Ala (A)  18	  7.6%
Arg (R)  16	  6.7%
Asn (N)  18	  7.6%
Asp (D)  19	  8.0%
Cys (C)   4	  1.7%
Gln (Q)   8	  3.4%
Glu (E)  19	  8.0%
Gly (G)   3	  1.3%
His (H)   5	  2.1%
Ile (I)   6	  2.5%
Leu (L)  27	 11.3%
Lys (K)  11	  4.6%
Met (M)   5	  2.1%
Phe (F)  11	  4.6%
Pro (P)  13	  5.5%
Ser (S)  29	 12.2%
Thr (T)  17	  7.1%
Trp (W)   2	  0.8%
Tyr (Y)   2	  0.8%
Val (V)   5	  2.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1154
Hydrogen    H	      1825
Nitrogen    N	       335
Oxygen      O	       389
Sulfur      S	         9

Formula: C1154H1825N335O389S9
Total number of atoms: 3712

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14230
Abs 0.1% (=1 g/l)   0.529, assuming all pairs of Cys residues form cystines


Ext. coefficient    13980
Abs 0.1% (=1 g/l)   0.520, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.79
This classifies the protein as unstable.



Aliphatic index: 67.73

Grand average of hydropathicity (GRAVY): -0.769