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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY ITYALSGYVR

70 80
SRGESDDSLN RLAQNDGLLK NVWSYSR


References and documentation are available.

Number of amino acids: 87 Molecular weight: 9759.87 Theoretical pI: 5.75
Amino acid composition: 
Ala (A) 7 8.0% Arg (R) 7 8.0% Asn (N) 6 6.9% Asp (D) 7 8.0% Cys (C) 1 1.1% Gln (Q) 3 3.4% Glu (E) 6 6.9% Gly (G) 6 6.9% His (H) 1 1.1% Ile (I) 5 5.7% Leu (L) 7 8.0% Lys (K) 5 5.7% Met (M) 1 1.1% Phe (F) 0 0.0% Pro (P) 2 2.3% Ser (S) 8 9.2% Thr (T) 2 2.3% Trp (W) 1 1.1% Tyr (Y) 5 5.7% Val (V) 7 8.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 13 Total number of positively charged residues (Arg + Lys): 12 Atomic composition: Carbon C 421 Hydrogen H 675 Nitrogen N 125 Oxygen O 138 Sulfur S 2 Formula: C421H675N125O138S2 Total number of atoms: 1361 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 12950 Abs 0.1% (=1 g/l) 1.327, assuming all pairs of Cys residues form cystines Ext. coefficient 12950 Abs 0.1% (=1 g/l) 1.327, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 18.28 This classifies the protein as stable. Aliphatic index: 85.17 Grand average of hydropathicity (GRAVY): -0.649