ProtParam
User-provided sequence:
10 20 30 40 50 60
MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY ITYALSGYVR
70 80
SRGESDDSLN RLAQNDGLLK NVWSYSR
References and
documentation are available.
Number of amino acids: 87
Molecular weight: 9759.87
Theoretical pI: 5.75
Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 12
Atomic composition:
Carbon C 421
Hydrogen H 675
Nitrogen N 125
Oxygen O 138
Sulfur S 2
Formula: C
421H
675N
125O
138S
2
Total number of atoms: 1361
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 1.327, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 1.327, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 18.28
This classifies the protein as stable.
Aliphatic index: 85.17
Grand average of hydropathicity (GRAVY): -0.649