ProtParam
User-provided sequence:
10 20 30 40 50 60
MENESSESRN RARLAIMELA NMISVPMSLN AAVRLGIADA IWNGGANSPL SAAEILPRLH
70 80 90 100 110 120
LPSHTTIGGD PENLQRILRM LTSYGVFSEH LVGSIERKYS LTDVGKTLVT DSGGLSYAAY
130 140 150 160 170 180
VLQHHQEALM RAWPLVHTAV VEPETEPYVK ANGEAAYAQY GKSEEMNGLM QKAMSGVSVP
190 200 210 220 230 240
FMKAILDGYD GFKSVDILVD VGGSAGDCLR MILQQFPNVR EGINFDLPEV VAKAPNIPGV
250 260 270 280 290 300
THVGGDMFQS VPSADAIFMK WVLTTWTDEE CKQIMKNCYN ALPVGGKLIA CEPVLPKETD
310 320 330 340 350
ESHRTRALLE GDIFVMTIYR TKGKHRTEEE FIELGLSAGF PTFRPFYIDY FYTILEFQK
References and
documentation are available.
Number of amino acids: 359
Molecular weight: 39688.48
Theoretical pI: 5.28
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1772
Hydrogen H 2773
Nitrogen N 469
Oxygen O 527
Sulfur S 19
Formula: C
1772H
2773N
469O
527S
19
Total number of atoms: 5560
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41620
Abs 0.1% (=1 g/l) 1.049, assuming all pairs of Cys residues form cystines
Ext. coefficient 41370
Abs 0.1% (=1 g/l) 1.042, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.19
This classifies the protein as unstable.
Aliphatic index: 88.02
Grand average of hydropathicity (GRAVY): -0.096