ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MENGKCNGAT TVKLPEIKFT KLFINGQFID AASGKTFETI DPRNGEVIAT IAEGDKEDVD 

        70         80         90        100        110        120 
LAVNAARYAF DHGPWPRMTG FERAKLINKF ADLIEENIEE LAKLDAVDGG KLFQLGKYAD 

       130        140        150        160        170        180 
IPATAGHFRY NAGAADKIHG ETLKMTRQSL FGYTLKEPIG VVGNIIPWNF PSIMFATKVA 

       190        200        210        220        230        240 
PAMAAGCTMV VKPAEQTSLS ALFYAHLSKE AGIPDGVLNI VTGFGSTAGA AIASHMDVDK 

       250        260        270        280        290        300 
VSFTGSTDVG RKIMQAAAAS NLKKVSLELG GKSPLLIFND ADIDKAADLA LLGCFYNKGE 

       310        320        330        340        350        360 
ICVASSRVFV QEGIYDKVVE KLVEKAKDWT VGDPFDSTAR QGPQVDKRQF EKILSYIEHG 

       370        380        390        400        410        420 
KNEGATLLTG GKAIGDKGYF IQPTIFADVT EDMKIYQDEI FGPVMSLMKF KTVEEGIKCA 

       430        440        450        460        470        480 
NNTKYGLAAG ILSQDIDLIN TVSRSIKAGI IWVNCYFGFD LDCPYGGYKM SGNCRESGMD 

       490        500 
ALDNYLQTKS VVMPLHNSPW M 

References and documentation are available.

Number of amino acids: 501

Molecular weight: 54360.29

Theoretical pI: 5.47

Amino acid composition: 
Ala (A)  51	 10.2%
Arg (R)  12	  2.4%
Asn (N)  22	  4.4%
Asp (D)  34	  6.8%
Cys (C)   8	  1.6%
Gln (Q)  13	  2.6%
Glu (E)  28	  5.6%
Gly (G)  48	  9.6%
His (H)   7	  1.4%
Ile (I)  37	  7.4%
Leu (L)  36	  7.2%
Lys (K)  41	  8.2%
Met (M)  15	  3.0%
Phe (F)  26	  5.2%
Pro (P)  19	  3.8%
Ser (S)  25	  5.0%
Thr (T)  28	  5.6%
Trp (W)   5	  1.0%
Tyr (Y)  15	  3.0%
Val (V)  31	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2436
Hydrogen    H	      3818
Nitrogen    N	       632
Oxygen      O	       729
Sulfur      S	        23

Formula: C2436H3818N632O729S23
Total number of atoms: 7638

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50350
Abs 0.1% (=1 g/l)   0.926, assuming all pairs of Cys residues form cystines


Ext. coefficient    49850
Abs 0.1% (=1 g/l)   0.917, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.60
This classifies the protein as stable.



Aliphatic index: 84.95

Grand average of hydropathicity (GRAVY): -0.085