ProtParam
User-provided sequence:
10 20 30 40 50 60
MENKETKQEP AAAAEQKTVP LIEDEIERSK VGIMRALCDR QDPETKEVDD LMIRRFLRAR
70 80 90 100 110 120
DLDIEKASTM FLNYLTWKRS MLPKGHIPEA EIANDLSHNK MCMQGHDKMG RPIAVAIGNR
130 140 150 160 170 180
HNPSKGNPDE FKRFVVYTLE KICARMPRGQ EKFVAIGDLQ GWGYSNCDIR GYLAALSTLQ
190 200 210 220 230 240
DCYPERLGKL YIVHAPYIFM TAWKVIYPFI DANTKKKIVF VENKKLTPTL LEDIDESQLP
250
DIYGGKLPLV PIQET
References and
documentation are available.
Number of amino acids: 255
Molecular weight: 29215.84
Theoretical pI: 6.96
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1302
Hydrogen H 2074
Nitrogen N 354
Oxygen O 378
Sulfur S 15
Formula: C
1302H
2074N
354O
378S
15
Total number of atoms: 4123
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30160
Abs 0.1% (=1 g/l) 1.032, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 1.024, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.79
This classifies the protein as unstable.
Aliphatic index: 84.94
Grand average of hydropathicity (GRAVY): -0.453