ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MENKLKLVVV GDTYTGKTSL LVAYTKKQFL DNYTTTVFDN WAVSVHIDNK NYAVNLFDTA 

        70         80         90        100        110        120 
GQGNYEQIRC LSYPHANVFL VCFSMIDRKT LESCRSTWIP EIRKYAGDNV PIMLVGTKND 

       130        140        150        160        170        180 
LVDDADSRNT VTEDYAKRVA HEIGCHKFYS CSALTHKGLK RVFDESFLAA VGVKLEEETP 


NPCCTIL 

References and documentation are available.

Number of amino acids: 187

Molecular weight: 21107.06

Theoretical pI: 6.20

Amino acid composition: 
Ala (A)  12	  6.4%
Arg (R)   7	  3.7%
Asn (N)  12	  6.4%
Asp (D)  13	  7.0%
Cys (C)   7	  3.7%
Gln (Q)   3	  1.6%
Glu (E)  10	  5.3%
Gly (G)   9	  4.8%
His (H)   5	  2.7%
Ile (I)   8	  4.3%
Leu (L)  16	  8.6%
Lys (K)  14	  7.5%
Met (M)   3	  1.6%
Phe (F)   8	  4.3%
Pro (P)   5	  2.7%
Ser (S)  10	  5.3%
Thr (T)  16	  8.6%
Trp (W)   2	  1.1%
Tyr (Y)   9	  4.8%
Val (V)  18	  9.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon      C	       939
Hydrogen    H	      1465
Nitrogen    N	       249
Oxygen      O	       284
Sulfur      S	        10

Formula: C939H1465N249O284S10
Total number of atoms: 2947

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24785
Abs 0.1% (=1 g/l)   1.174, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   1.156, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.33
This classifies the protein as unstable.



Aliphatic index: 84.39

Grand average of hydropathicity (GRAVY): -0.213