ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MENLISLVNK IQRACTALGD HGDSSALPTL WDSLPAIAVV GGQSSGKSSV LESIVGKDFL 

        70         80         90        100        110        120 
PRGSGIVTRR PLVLQLQKID DGTREYAEFL HLPRKKFTDF AAVRKEIQDE TDRETGRSKA 

       130        140        150        160        170        180 
ISSVPIHLSI YSPNVVNLTL IDLPGLTKVA VDGQSDSIVK DIENMVRSYI EKPNCIILAI 

       190        200        210        220        230        240 
SPANQDLATS DAIKISREVD PSGDRTFGVL TKIDLMDKGT DAVEILEGRS FKLKYPWVGV 

       250        260        270        280        290        300 
VNRSQADINK NVDMIAARKR EREYFSNTTE YRHLANKMGS EHLAKMLSKH LERVIKSRIP 

       310        320        330        340        350        360 
GIQSLINKTV LELETELSRL GKPIAADAGG KLYSIMEICR LFDQIFKEHL DGVRAGGEKV 

       370        380        390        400        410        420 
YNVFDNQLPA ALKRLQFDKQ LAMDNIRKLV TEADGYQPHL IAPEQGYRRL IESSIVSIRG 

       430        440        450        460        470        480 
PAEASVDTVH AILKDLVHKS VNETVELKQY PALRVEVTNA AIESLDKMRE GSKKATLQLV 

       490        500        510        520        530        540 
DMECSYLTVD FFRKLPQDVE KGGNPTHSIF DRYNDSYLRR IGSNVLSYVN MVCAGLRNSI 

       550        560        570        580        590        600 
PKSIVYCQVR EAKRSLLDHF FAELGTMDMK RLSSLLNEDP AIMERRSAIS KRLELYRAAQ 

       610 
SEIDAVAWSK 

References and documentation are available.

Number of amino acids: 610

Molecular weight: 68172.28

Theoretical pI: 8.47

Amino acid composition: 
Ala (A)  45	  7.4%
Arg (R)  42	  6.9%
Asn (N)  24	  3.9%
Asp (D)  41	  6.7%
Cys (C)   6	  1.0%
Gln (Q)  20	  3.3%
Glu (E)  40	  6.6%
Gly (G)  34	  5.6%
His (H)  12	  2.0%
Ile (I)  45	  7.4%
Leu (L)  63	 10.3%
Lys (K)  43	  7.0%
Met (M)  14	  2.3%
Phe (F)  16	  2.6%
Pro (P)  23	  3.8%
Ser (S)  52	  8.5%
Thr (T)  25	  4.1%
Trp (W)   3	  0.5%
Tyr (Y)  17	  2.8%
Val (V)  45	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 85

Atomic composition:

Carbon      C	      3007
Hydrogen    H	      4894
Nitrogen    N	       850
Oxygen      O	       911
Sulfur      S	        20

Formula: C3007H4894N850O911S20
Total number of atoms: 9682

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42205
Abs 0.1% (=1 g/l)   0.619, assuming all pairs of Cys residues form cystines


Ext. coefficient    41830
Abs 0.1% (=1 g/l)   0.614, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.78
This classifies the protein as unstable.



Aliphatic index: 97.82

Grand average of hydropathicity (GRAVY): -0.276