ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MENQEKPLSV DEEYDLWKSN VPLMYDFVSE TRLTWPSLTV QWLPTPVQEL DGGFIKQELI 

        70         80         90        100        110        120 
IGTHTSGEEE NYLKFAEINL PKEILSNEDP QEEAGEEYQS SLPAPRSNIR ITAKYEHEEE 

       130        140        150        160        170        180 
ITRARYMPQD PNIVATINGQ GTVFLYSRSE GLQSTLKFHK DNGYALSFST LVKGRLLSGS 

       190        200        210        220        230        240 
DDHTVALWEV GSGGDPTKPV RTWNDLHSDI INDNKWHNFN KDLFGTVSED SLLKINDVRA 

       250        260        270        280        290        300 
NNTTIDTVKC PQPFNTLAFS HHSSNLLAAA GMDSYVYLYD LRNMKEPLHH MSGHEDAVNN 

       310        320        330        340        350        360 
LEFSTHVDGV VVSSGSDNRL MMWDLKQIGA EQTPDDAEDG VPELIMVHAG HRSSVNDFDL 

       370        380        390        400 
NPQIPWLVAS AEEENILQVW KCSHSLPIVG GPPKVNKDII S 

References and documentation are available.

Number of amino acids: 401

Molecular weight: 45060.15

Theoretical pI: 4.72

Amino acid composition: 
Ala (A)  19	  4.7%
Arg (R)  12	  3.0%
Asn (N)  27	  6.7%
Asp (D)  29	  7.2%
Cys (C)   2	  0.5%
Gln (Q)  14	  3.5%
Glu (E)  33	  8.2%
Gly (G)  24	  6.0%
His (H)  15	  3.7%
Ile (I)  21	  5.2%
Leu (L)  38	  9.5%
Lys (K)  19	  4.7%
Met (M)   9	  2.2%
Phe (F)  12	  3.0%
Pro (P)  23	  5.7%
Ser (S)  35	  8.7%
Thr (T)  22	  5.5%
Trp (W)   9	  2.2%
Tyr (Y)  11	  2.7%
Val (V)  27	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1994
Hydrogen    H	      3066
Nitrogen    N	       536
Oxygen      O	       635
Sulfur      S	        11

Formula: C1994H3066N536O635S11
Total number of atoms: 6242

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66015
Abs 0.1% (=1 g/l)   1.465, assuming all pairs of Cys residues form cystines


Ext. coefficient    65890
Abs 0.1% (=1 g/l)   1.462, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.36
This classifies the protein as unstable.



Aliphatic index: 81.65

Grand average of hydropathicity (GRAVY): -0.515