ProtParam
User-provided sequence:
10 20 30 40 50 60
MENQLAKSIE ERTFQYQDSL PPLPVPSLEE SLKKYLESVK PFANEDEYKK TEEIVQKFQD
70 80 90 100 110 120
GVGKTLHQKL LERAKGKRNW LEEWWLNVAY LDVRIPSQLN VNFVGPSPHF EHYWPAREGT
130 140 150 160 170 180
QLERGSILLW HNLNYWQLLR REKLPVHKSG NTPLDMNQFR MLFSTCKVPG ITRDSIMNYF
190 200 210 220 230 240
KTESEGHCPT HIAVLCRGRA FVFDVLHDGC LITPPELLRQ LTYIYQKCWN EPVGPSIAAL
250 260 270 280 290 300
TSEERTRWAK AREYLIGLDP ENLTLLEKIQ SSLFVYSIED TSPHATPENF SQVFEMLLGG
310 320 330 340 350 360
DPAVRWGDKS YNLISFANGI FGCSCDHAPY DAMVMVNIAH YVDEKLLETE GRWKGSEKVR
370 380 390 400 410 420
DIPLPEELAF TVDEKILNDV YQAKAQHLKA ASDLQIAAST FTSFGKKLTK KEALHPDTFI
430 440 450 460 470 480
QLALQLAYYR LHGRPGCCYE TAMTRYFYHG RTETVRSCTV EAVRWCQSMQ DPSASLLERQ
490 500 510 520 530 540
QKMLDAFAKH NKMMRDCSHG KGFDRHLLGL LLIAKEEGLP VPELFEDPLF SRSGGGGNFV
550 560 570 580 590 600
LSTSLVGYLR IQGVVVPMVH NGYGFFYHIR DDRFVVTCSS WRSCLETDAE KLVEMIFHAF
610
HDMIHLMNTA HL
References and
documentation are available.
Number of amino acids: 612
Molecular weight: 70288.46
Theoretical pI: 6.26
Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 68
Atomic composition:
Carbon C 3164
Hydrogen H 4872
Nitrogen N 852
Oxygen O 905
Sulfur S 30
Formula: C
3164H
4872N
852O
905S
30
Total number of atoms: 9823
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 101145
Abs 0.1% (=1 g/l) 1.439, assuming all pairs of Cys residues form cystines
Ext. coefficient 100270
Abs 0.1% (=1 g/l) 1.427, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.85
This classifies the protein as unstable.
Aliphatic index: 82.84
Grand average of hydropathicity (GRAVY): -0.342