ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MENQLAKSIE ERTFQYQDSL PPLPVPSLEE SLKKYLESVK PFANEDEYKK TEEIVQKFQD 

        70         80         90        100        110        120 
GVGKTLHQKL LERAKGKRNW LEEWWLNVAY LDVRIPSQLN VNFVGPSPHF EHYWPAREGT 

       130        140        150        160        170        180 
QLERGSILLW HNLNYWQLLR REKLPVHKSG NTPLDMNQFR MLFSTCKVPG ITRDSIMNYF 

       190        200        210        220        230        240 
KTESEGHCPT HIAVLCRGRA FVFDVLHDGC LITPPELLRQ LTYIYQKCWN EPVGPSIAAL 

       250        260        270        280        290        300 
TSEERTRWAK AREYLIGLDP ENLTLLEKIQ SSLFVYSIED TSPHATPENF SQVFEMLLGG 

       310        320        330        340        350        360 
DPAVRWGDKS YNLISFANGI FGCSCDHAPY DAMVMVNIAH YVDEKLLETE GRWKGSEKVR 

       370        380        390        400        410        420 
DIPLPEELAF TVDEKILNDV YQAKAQHLKA ASDLQIAAST FTSFGKKLTK KEALHPDTFI 

       430        440        450        460        470        480 
QLALQLAYYR LHGRPGCCYE TAMTRYFYHG RTETVRSCTV EAVRWCQSMQ DPSASLLERQ 

       490        500        510        520        530        540 
QKMLDAFAKH NKMMRDCSHG KGFDRHLLGL LLIAKEEGLP VPELFEDPLF SRSGGGGNFV 

       550        560        570        580        590        600 
LSTSLVGYLR IQGVVVPMVH NGYGFFYHIR DDRFVVTCSS WRSCLETDAE KLVEMIFHAF 

       610 
HDMIHLMNTA HL 

References and documentation are available.

Number of amino acids: 612

Molecular weight: 70288.46

Theoretical pI: 6.26

Amino acid composition: 
Ala (A)  36	  5.9%
Arg (R)  33	  5.4%
Asn (N)  22	  3.6%
Asp (D)  29	  4.7%
Cys (C)  14	  2.3%
Gln (Q)  24	  3.9%
Glu (E)  48	  7.8%
Gly (G)  36	  5.9%
His (H)  24	  3.9%
Ile (I)  25	  4.1%
Leu (L)  69	 11.3%
Lys (K)  35	  5.7%
Met (M)  16	  2.6%
Phe (F)  30	  4.9%
Pro (P)  31	  5.1%
Ser (S)  38	  6.2%
Thr (T)  31	  5.1%
Trp (W)  12	  2.0%
Tyr (Y)  23	  3.8%
Val (V)  36	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      3164
Hydrogen    H	      4872
Nitrogen    N	       852
Oxygen      O	       905
Sulfur      S	        30

Formula: C3164H4872N852O905S30
Total number of atoms: 9823

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   101145
Abs 0.1% (=1 g/l)   1.439, assuming all pairs of Cys residues form cystines


Ext. coefficient   100270
Abs 0.1% (=1 g/l)   1.427, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.85
This classifies the protein as unstable.



Aliphatic index: 82.84

Grand average of hydropathicity (GRAVY): -0.342