ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MENTLGEGST VNASVDVDQH GNDNNSDSNA NAAVAGVANT DTAGEESQQQ DESLKDEATV 

        70         80         90        100        110        120 
PNTRDAESEA ITVTAKQQPT MQANKLDSQE TPSTEESRAQ NVFGQDNEDS DNLFGETESS 

       130        140        150        160        170        180 
VSNNEANTPS IPTNPVDNEN NKPAIKEDST IQDSNGDVKN MEDVKIQKEE EPENNTVIEG 

       190        200        210        220        230        240 
VKEESQPDEN TKEMDEVEED DEDDDQPMIS PDNSIFGDTK SESKQLGNTS SVANTPSEIP 

       250        260        270        280        290        300 
DAHKAEQEDI IEKTESVDKK VDSGEERNEQ EREIMNDHSK SANPKKTTIT RVEPETFEIP 

       310        320        330        340        350        360 
QAHEIVIPSY SKWFNLEKIH SIEVQSLPEF FTNRIPSKTP EVYMRYRNFM VNSYRLNPNE 

       370        380        390        400        410        420 
YFSVTTARRN VSGDAAALFR LHKFLTKWGL INYQVDSKLL PKNIEPPLTS QYSTRHDAPR 

       430        440        450        460        470        480 
GLFPFESYKP SVQLPDMAKL KKMMNTSDSE STLYKYLKES KRKYDEITHP PSTTDDENGD 

       490        500        510        520        530        540 
KNDNGGKMNN EVSTSTSMTG DANLLEEGET SRPLKKVKIL EQIDENWSKE DLQKLLKGIQ 

       550        560        570        580        590        600 
EFGADWYKVA KNVGNKSPEQ CILRFLQLPI EDKFLYGDGN GKGDNDNGLG PLKYAPHLPF 

       610        620        630        640        650        660 
SKSENPVLST IAFLVGLVNP KTVQSMTQRA IQSAESIKSQ KEEISDQKPI EHIKEGSEIA 

       670        680        690        700        710        720 
ISSLGYRSHI FATNEERQMN FLTNELIRLQ MEKLDAKLNH LKKLEKFMEL ERKTLERQQE 

       730        740        750        760        770        780 
NLLIQRLNFN QNSSKIVNVL SKCLNLISDS NINNSSVAEK EEIRSQIDHF KSMLSKPETL 

       790        800        810        820 
SIGKNPFNKP NIETGENHNG QSISNENDVK PISIEAPQFY RYWSA 

References and documentation are available.

Number of amino acids: 825

Molecular weight: 92926.38

Theoretical pI: 4.78

Amino acid composition: 
Ala (A)  39	  4.7%
Arg (R)  26	  3.2%
Asn (N)  75	  9.1%
Asp (D)  54	  6.5%
Cys (C)   2	  0.2%
Gln (Q)  41	  5.0%
Glu (E)  91	 11.0%
Gly (G)  35	  4.2%
His (H)  14	  1.7%
Ile (I)  48	  5.8%
Leu (L)  55	  6.7%
Lys (K)  68	  8.2%
Met (M)  18	  2.2%
Phe (F)  25	  3.0%
Pro (P)  44	  5.3%
Ser (S)  80	  9.7%
Thr (T)  49	  5.9%
Trp (W)   5	  0.6%
Tyr (Y)  17	  2.1%
Val (V)  39	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 145
Total number of positively charged residues (Arg + Lys): 94

Atomic composition:

Carbon      C	      4009
Hydrogen    H	      6348
Nitrogen    N	      1120
Oxygen      O	      1378
Sulfur      S	        20

Formula: C4009H6348N1120O1378S20
Total number of atoms: 12875

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52955
Abs 0.1% (=1 g/l)   0.570, assuming all pairs of Cys residues form cystines


Ext. coefficient    52830
Abs 0.1% (=1 g/l)   0.569, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.89
This classifies the protein as unstable.



Aliphatic index: 67.13

Grand average of hydropathicity (GRAVY): -0.947