ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MENTTNRNTA GVLTSSNGNF ATNSVAASTP KRSKSARRKT FKCTGYDGCT MSFTRAEHLA 

        70         80         90        100        110        120 
RHIRKHTGEK PFQCPACLKF FSRVDNLKQH RESVHAHKNH HSTSSHQRKP SSSSLSSSSS 

       130        140        150        160        170        180 
ASSSSSASSS TSYSDPYRKT NINSGNMPMM AENEKAPQII HSSPEFITST RSIPPISPRS 

       190        200        210        220        230        240 
IYNTQRQQQH QQQQHQQAPY YFPSHPITDS YYQYPLPSNN NTINYLPSVD VQYPLNVSPS 

       250        260        270        280        290        300 
STSHPASEVI ISSFPPRSMP STSFKYKDSA DFQARTTMNK YNIRPSNINV NTSNINNHLD 

       310        320        330        340        350        360 
SFSPPFSPST TVAEAKPIIL PQYQQAFSQP PNGNKNNNMS SSKNGGKGGE NFKNTDDRND 

       370        380        390 
NNNKKRSETL SESDISVNTN KKRLSVDYIL T 

References and documentation are available.

Number of amino acids: 391

Molecular weight: 43517.81

Theoretical pI: 9.77

Amino acid composition: 
Ala (A)  20	  5.1%
Arg (R)  20	  5.1%
Asn (N)  40	 10.2%
Asp (D)  13	  3.3%
Cys (C)   4	  1.0%
Gln (Q)  21	  5.4%
Glu (E)  12	  3.1%
Gly (G)  11	  2.8%
His (H)  15	  3.8%
Ile (I)  19	  4.9%
Leu (L)  13	  3.3%
Lys (K)  24	  6.1%
Met (M)   8	  2.0%
Phe (F)  15	  3.8%
Pro (P)  31	  7.9%
Ser (S)  68	 17.4%
Thr (T)  30	  7.7%
Trp (W)   0	  0.0%
Tyr (Y)  15	  3.8%
Val (V)  12	  3.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      1866
Hydrogen    H	      2914
Nitrogen    N	       566
Oxygen      O	       616
Sulfur      S	        12

Formula: C1866H2914N566O616S12
Total number of atoms: 5974

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22600
Abs 0.1% (=1 g/l)   0.519, assuming all pairs of Cys residues form cystines


Ext. coefficient    22350
Abs 0.1% (=1 g/l)   0.514, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 66.82
This classifies the protein as unstable.



Aliphatic index: 45.93

Grand average of hydropathicity (GRAVY): -1.005