ProtParam
User-provided sequence:
10 20 30 40 50 60
MEPASKKSKM GKNVKFNNNK KKYFHAKRQT LQPGQRGFFA TCNINEKACV RECYNLLNHY
70 80 90 100 110 120
ADILYGSEKP ENEPEKKQPE EGAGGDAGED DPKPAAGGTS DDDDDLEAAA AKCREMLSQR
130 140 150 160 170 180
KMRFQNVDTN TTNCVFIRTQ LEDPVALGKH IINDIATTGK SMSRFVLRLV PIEVVCRANM
190 200 210 220 230 240
PDIITAAGEL FDKHFLKEPT SYGIIFNHRY NQQIKRDQII TQLAELVNSK NVGNKVDLKE
250 260 270 280 290 300
AKKSIIVEVL RGWCLLSVID NYLECKKFNL AELANPSDKK SSGEGDSKSE TSEVANGNDK
310 320
EQAESSEESK SNDDENKDST ENDK
References and
documentation are available.
Number of amino acids: 324
Molecular weight: 36206.52
Theoretical pI: 5.62
Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 1559
Hydrogen H 2498
Nitrogen N 448
Oxygen O 515
Sulfur S 14
Formula: C
1559H
2498N
448O
515S
14
Total number of atoms: 5034
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 16430
Abs 0.1% (=1 g/l) 0.454, assuming all pairs of Cys residues form cystines
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.440, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.08
This classifies the protein as unstable.
Aliphatic index: 67.47
Grand average of hydropathicity (GRAVY): -0.839