ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPASKKSKM GKNVKFNNNK KKYFHAKRQT LQPGQRGFFA TCNINEKACV RECYNLLNHY 

        70         80         90        100        110        120 
ADILYGSEKP ENEPEKKQPE EGAGGDAGED DPKPAAGGTS DDDDDLEAAA AKCREMLSQR 

       130        140        150        160        170        180 
KMRFQNVDTN TTNCVFIRTQ LEDPVALGKH IINDIATTGK SMSRFVLRLV PIEVVCRANM 

       190        200        210        220        230        240 
PDIITAAGEL FDKHFLKEPT SYGIIFNHRY NQQIKRDQII TQLAELVNSK NVGNKVDLKE 

       250        260        270        280        290        300 
AKKSIIVEVL RGWCLLSVID NYLECKKFNL AELANPSDKK SSGEGDSKSE TSEVANGNDK 

       310        320 
EQAESSEESK SNDDENKDST ENDK 

References and documentation are available.

Number of amino acids: 324

Molecular weight: 36206.52

Theoretical pI: 5.62

Amino acid composition: 
Ala (A)  24	  7.4%
Arg (R)  13	  4.0%
Asn (N)  27	  8.3%
Asp (D)  24	  7.4%
Cys (C)   8	  2.5%
Gln (Q)  12	  3.7%
Glu (E)  30	  9.3%
Gly (G)  19	  5.9%
His (H)   5	  1.5%
Ile (I)  17	  5.2%
Leu (L)  21	  6.5%
Lys (K)  35	 10.8%
Met (M)   6	  1.9%
Phe (F)  11	  3.4%
Pro (P)  12	  3.7%
Ser (S)  22	  6.8%
Thr (T)  14	  4.3%
Trp (W)   1	  0.3%
Tyr (Y)   7	  2.2%
Val (V)  16	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 48

Atomic composition:

Carbon      C	      1559
Hydrogen    H	      2498
Nitrogen    N	       448
Oxygen      O	       515
Sulfur      S	        14

Formula: C1559H2498N448O515S14
Total number of atoms: 5034

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16430
Abs 0.1% (=1 g/l)   0.454, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.440, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.08
This classifies the protein as unstable.



Aliphatic index: 67.47

Grand average of hydropathicity (GRAVY): -0.839