ProtParam
User-provided sequence:
10 20 30 40 50 60
MEPAVSLAVC ALLFLLWVRV KGLEFVLIHQ RWVFVCLFLL PLSLIFDIYY YVRAWVVFKL
70 80 90 100 110 120
SSAPRLHEQR VQDIQKQVRE WKEQGSKTFM CTGRPGWLTV SLRVGKYKKT HKNIMINLMD
130 140 150 160 170 180
ILEVDTKKQI VRVEPLVSMG QVTALLNSIG WTLPVLPELD DLTVGGLIMG TGIESSSHKY
190 200 210 220 230 240
GLFQHICTAY ELILADGSFV RCTPSENSDL FYAVPWSCGT LGFLVAAEIR IIPAKKYVKL
250 260 270 280 290 300
RFEPVRGLEA ICEKFTHESQ RLENHFVEGL LYSLDEAVIM TGVMTDDVEP SKLNSIGSYY
310 320 330 340 350 360
KPWFFKHVEN YLKTNREGLE YIPLRHYYHR HTRSIFWELQ DIIPFGNNPI FRYLFGWMVP
370 380 390 400 410 420
PKISLLKLTQ GETLRKLYEQ HHVVQDMLVP MKCLSQALHT FQNDIHVYPI WLCPFILPSQ
430 440 450 460 470 480
PGLVHPKGDE AELYVDIGAY GEPRVKHFEA RSCMRQLEKF VRSVHGFQML YADCYMNREE
490 500 510
FWEMFDGSLY HKLRKQLGCQ DAFPEVYDKI CKAARH
References and
documentation are available.
Number of amino acids: 516
Molecular weight: 60047.14
Theoretical pI: 8.12
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 2763
Hydrogen H 4247
Nitrogen N 713
Oxygen O 731
Sulfur S 28
Formula: C
2763H
4247N
713O
731S
28
Total number of atoms: 8482
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 102510
Abs 0.1% (=1 g/l) 1.707, assuming all pairs of Cys residues form cystines
Ext. coefficient 101760
Abs 0.1% (=1 g/l) 1.695, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.68
This classifies the protein as unstable.
Aliphatic index: 96.65
Grand average of hydropathicity (GRAVY): -0.045