ProtParam
User-provided sequence:
10 20 30 40 50 60
MEPDNSPRKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEI DEDRIPNSLL
70 80 90 100 110 120
KSTLSMSPRQ RKKMTRTTPT MKELQTMVEH HLGQQKQGEE PEGATESTGN QESCPPGIPD
130 140 150 160 170
TGSASRPDTP GTAQKSAESN PKTQEQCGVE PRTEDSSAHM LPLDSQGASL V
References and
documentation are available.
Number of amino acids: 171
Molecular weight: 18717.76
Theoretical pI: 5.15
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 19
Atomic composition:
Carbon C 785
Hydrogen H 1287
Nitrogen N 237
Oxygen O 276
Sulfur S 8
Formula: C
785H
1287N
237O
276S
8
Total number of atoms: 2593
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.007, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 66.69
This classifies the protein as unstable.
Aliphatic index: 54.80
Grand average of hydropathicity (GRAVY): -1.039