ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPEDTQLKE DIKTTVNYIK QHGVEFENKL LEDERFSFIK KDDPLHEYYT KLMNEPTDTV 

        70         80         90        100        110        120 
SGEDNDRKSE REIARPPDFL FSQYDTGISR RDMEVIKLTA RYYAKDKSIV EQMISKDGEA 

       130        140        150        160        170        180 
RLNFMNSSHP LHKTFTDFVA QYKRVYSFTG QEIKKSKRTI LDNCFERTQY WEFEKDKDRE 

       190        200        210        220        230        240 
HDKLVELCKI QFAAIPWDKF TQVAKFSIPE DTEIFEGSLD LEQMRLRRVQ TGIKLFDSIK 

       250        260        270        280 
PTNEEEKIVS DQGKQKGGDS KGKKRKIRAV GETRLKKSKK 

References and documentation are available.

Number of amino acids: 280

Molecular weight: 33052.41

Theoretical pI: 7.67

Amino acid composition: 
Ala (A)   9	  3.2%
Arg (R)  18	  6.4%
Asn (N)   8	  2.9%
Asp (D)  24	  8.6%
Cys (C)   2	  0.7%
Gln (Q)  13	  4.6%
Glu (E)  29	 10.4%
Gly (G)  12	  4.3%
His (H)   5	  1.8%
Ile (I)  18	  6.4%
Leu (L)  17	  6.1%
Lys (K)  36	 12.9%
Met (M)   6	  2.1%
Phe (F)  16	  5.7%
Pro (P)   9	  3.2%
Ser (S)  17	  6.1%
Thr (T)  18	  6.4%
Trp (W)   2	  0.7%
Tyr (Y)   9	  3.2%
Val (V)  12	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      1464
Hydrogen    H	      2317
Nitrogen    N	       403
Oxygen      O	       452
Sulfur      S	         8

Formula: C1464H2317N403O452S8
Total number of atoms: 4644

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24535
Abs 0.1% (=1 g/l)   0.742, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.739, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.46
This classifies the protein as stable.



Aliphatic index: 64.39

Grand average of hydropathicity (GRAVY): -1.007