ProtParam
User-provided sequence:
10 20 30 40 50 60
MEPESIGDVG NHAQDDSASI VSGPRRRSTS KTSSAKNIRN SSNISPASMI FRNLLILEDD
70 80 90 100 110 120
LRRQAHEQKI LKWQFTLFLA SMAGVGAFTF YELYFTSDYV KGLHRVILQF TLSFISITVV
130 140 150 160 170 180
LFHISGQYRR TIVIPRRFFT STNKGIRQFN VKLVKVQSTW DEKYTDSVRF VSRTIAYCNI
190 200 210 220 230 240
YCLKKFLWLK DDNAIVKFWK SVTIQSQPRI GAVDVKLVLN PRAFSAEIRE GWEIYRDEFW
250
AREGARRRKQ AHELRPKSE
References and
documentation are available.
Number of amino acids: 259
Molecular weight: 30113.60
Theoretical pI: 10.03
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1362
Hydrogen H 2137
Nitrogen N 385
Oxygen O 378
Sulfur S 5
Formula: C
1362H
2137N
385O
378S
5
Total number of atoms: 4267
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45045
Abs 0.1% (=1 g/l) 1.496, assuming all pairs of Cys residues form cystines
Ext. coefficient 44920
Abs 0.1% (=1 g/l) 1.492, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.83
This classifies the protein as unstable.
Aliphatic index: 86.56
Grand average of hydropathicity (GRAVY): -0.321