ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPFAFGRGA PALCILTAAA RINLDNFVPC CWALFRLSFF FPLDPAYIRN ENKETRTSWI 

        70         80         90        100        110        120 
SIEFFFFVKH CLSQHTFFSK TLAPKRNFRA KKLKDIGDTR IDRADKDFLL VPEPSMFVNG 

       130        140        150        160        170        180 
NQSNFAKPAG QGILPIPKKS RIIKTDKPRP FLCPTCTRGF VRQEHLKRHQ HSHTREKPYL 

       190        200        210        220        230        240 
CIFCGRCFAR RDLVLRHQQK LHAALVGTGD PRRMTPAPNS TSSFASKRRH SVAADDPTDL 

       250        260        270        280        290        300 
HIIKIAGNKE TILPTPKNLA GKTSEELKEA VVALAKSNNV ELPVSAPVMN DKREKTPPSK 

       310        320        330        340        350        360 
AGSLGFREFK FSTKGVPVHS ASSDAVIDRA NTPSSMHKTK RHASFSASSA MTYMSSSNSP 

       370        380        390        400        410        420 
HHSITNFELV EDAPHQVGFS TPQMTAKQLM ESVSELDLPP LTLDEPPQAI KFNLNLFNND 

       430        440        450        460        470        480 
PSGQQQQQQQ QQQNSTSSTI VNSNNGSTVA TPGVYLLSSG PSLTDLLTMN SAHAGAGGYM 

       490        500        510        520        530        540 
SSHHSPFDLG CFSHDKPTVS EFNLPSSFPN TIPSNSTTAS NSYSNLANQT YRQMSNEQPL 

       550        560        570        580        590        600 
MSLSPKNPPT TVSDSSSTIN FNPGTNNLLE PSMEPNDKDS NIDPAAIDDK WLSEFINNSD 

       610        620        630        640        650        660 
PKSTFKINFN HFNDIGFIYS PPSSRSSIPN KSPPNHSATS LNHEKASLSP RLNLSLNGST 

       670        680        690        700        710        720 
DLPSTPQNQL KEPSYSDPIS HSSHKRRRDS VMMDYDLSNF FSSRQLDISK VLNGTEQNNS 

       730        740        750        760        770        780 
HVNDDVLTLS FPGETDSNAT QKQLPVLTPS DLLSPFSVPS VSQVLFTNEL RSMMLADNNI 

       790        800        810        820        830        840 
DSGAFPTTSQ LNDYVTYYKE EFHPFFSFIH LPSIIPNMDS YPLLLSISMV GALYGFHSTH 

       850        860        870        880        890        900 
AKVLANAAST QIRKSLKVSE KNPETTELWV IQTLVLLTFY CIFNKNTAVI KGMHGQLTTI 

       910        920        930        940        950        960 
IRLLKASRLN LPLESLCQPP IESDHIMEYE NSPHMFSKIR EQYNAPNQMN KNYQYFVLAQ 

       970        980        990       1000       1010       1020 
SRIRTCHAVL LISNLFSSLV GADCCFHSVD LKCGVPCYKE ELYQCRNSDE WSDLLCQYKI 

      1030       1040       1050       1060       1070       1080 
TLDSKFSLIE LSNGNEAYEN CLRFLSTGDS FFYGNARVSL STCLSLLISI HEKILIERNN 

      1090       1100       1110       1120       1130       1140 
ARISNNNTNS NNIELDDIEW KMTSRQRIDT MLKYWENLYL KNGGILTPTE NSMSTINANP 

      1150       1160       1170       1180       1190       1200 
AMRLIIPVYL FAKMRRCLDL AHVIEKIWLK DWSNMNKALE EVCYDMGSLR EATEYALNMV 

      1210       1220       1230       1240       1250       1260 
DAWTSFFTYI KQGKRRIFNT PVFATTCMFT AVLVISEYMK CVEDWARGYN ANNPNSALLD 

      1270       1280       1290       1300       1310       1320 
FSDRVLWLKA ERILRRLQMN LIPKECDVLK SYTDFLRWQD KDALDLSALN EEQAQRAMDP 

      1330       1340       1350       1360       1370       1380 
NTDINETIQL IVAASLSSKC LYLGVQILGD APIWPIILSF AHGLQSRAIY SVTKKRNTRI 

References and documentation are available.

Number of amino acids: 1380

Molecular weight: 155063.06

Theoretical pI: 8.27

Amino acid composition: 
Ala (A)  86	  6.2%
Arg (R)  64	  4.6%
Asn (N) 104	  7.5%
Asp (D)  70	  5.1%
Cys (C)  27	  2.0%
Gln (Q)  51	  3.7%
Glu (E)  63	  4.6%
Gly (G)  47	  3.4%
His (H)  38	  2.8%
Ile (I)  77	  5.6%
Leu (L) 137	  9.9%
Lys (K)  76	  5.5%
Met (M)  36	  2.6%
Phe (F)  70	  5.1%
Pro (P)  87	  6.3%
Ser (S) 154	 11.2%
Thr (T)  85	  6.2%
Trp (W)  14	  1.0%
Tyr (Y)  36	  2.6%
Val (V)  58	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 133
Total number of positively charged residues (Arg + Lys): 140

Atomic composition:

Carbon      C	      6866
Hydrogen    H	     10745
Nitrogen    N	      1893
Oxygen      O	      2077
Sulfur      S	        63

Formula: C6866H10745N1893O2077S63
Total number of atoms: 21644

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   132265
Abs 0.1% (=1 g/l)   0.853, assuming all pairs of Cys residues form cystines


Ext. coefficient   130640
Abs 0.1% (=1 g/l)   0.842, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.86
This classifies the protein as unstable.



Aliphatic index: 78.90

Grand average of hydropathicity (GRAVY): -0.374