ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPFDFFNSF KHALAVLKLP SKSMSTTDLK AFGERFAKSH TKFPAAPAMT KSILPNFSTV 

        70         80         90        100        110        120 
FLTAFSTCSK LRTSTFAIAK TASLSFANCE IPFAACSVLS WSLPTIAALQ PRRTKASVCT 

       130        140        150        160        170        180 
RHIVPAPPVT NATLPLNKSG LQEPSVTNLP SKVFAVFIVS MTRTYDLPNS KSVNIRNYEL 

       190        200 
LFKFRFSLIW SLTLIPILFG KLQ 

References and documentation are available.

Number of amino acids: 203

Molecular weight: 22418.37

Theoretical pI: 10.17

Amino acid composition: 
Ala (A)  20	  9.9%
Arg (R)   8	  3.9%
Asn (N)   9	  4.4%
Asp (D)   3	  1.5%
Cys (C)   4	  2.0%
Gln (Q)   3	  1.5%
Glu (E)   5	  2.5%
Gly (G)   3	  1.5%
His (H)   3	  1.5%
Ile (I)  10	  4.9%
Leu (L)  23	 11.3%
Lys (K)  15	  7.4%
Met (M)   4	  2.0%
Phe (F)  19	  9.4%
Pro (P)  16	  7.9%
Ser (S)  24	 11.8%
Thr (T)  19	  9.4%
Trp (W)   2	  1.0%
Tyr (Y)   2	  1.0%
Val (V)  11	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 8
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1034
Hydrogen    H	      1628
Nitrogen    N	       262
Oxygen      O	       277
Sulfur      S	         8

Formula: C1034H1628N262O277S8
Total number of atoms: 3209

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14230
Abs 0.1% (=1 g/l)   0.635, assuming all pairs of Cys residues form cystines


Ext. coefficient    13980
Abs 0.1% (=1 g/l)   0.624, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.66
This classifies the protein as unstable.



Aliphatic index: 88.97

Grand average of hydropathicity (GRAVY): 0.234