ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPGLSGERR SMAPLLEYER QQVLELLDSN GLVVCARGLG TDRLLYHFLR LHCHPACLVL 

        70         80         90        100        110        120 
VLNTQPAEEE YFINQLKIEG VEHLPRRVTN EIASNSRYEV YTQGGIIFAT SRILVVDFLT 

       130        140        150        160        170        180 
GRIPSDLITG ILVYRAHRII ESCQEAFILR LFRQKNKRGF IKAFTDNAVA FDTGFCHVER 

       190        200        210        220        230        240 
VMRNLFVRKL YLWPRFHVAV NSFLEQHKPE VVEIHVSMTP AMLAIQTAIL DILNACLKEL 

       250        260        270        280        290        300 
KCHNPSLEVE DLSLENALGK PFDKTIRHYL DPLWHQLGAK TKSLVQDLKI LRTLLQYLSQ 

       310        320        330        340        350        360 
YDCVTFLNLL ESLRATEKVF GQNSGWLFLD ASTSMFVNAR ARVYRVPDVK LNKKAKTSEK 

       370        380        390        400        410        420 
TSSPEVQETK KELVLESNPK WEALTDVLKE IEAENKESEA LGGPGRVLIC ASDDRTCCQL 

       430        440        450        460        470        480 
RDYLSAGAET FLLRLYRKTF EKDGKAEEVW VNVRKGDGPK RTTKSDKRPK AAPNKERASA 

       490        500        510        520        530        540 
KRGAPLKRKK QELTLTQVLG SAEEPPEDKA LEEDLCRQTS SSPEGCGVEI KRESFDLNVS 

       550        560        570        580        590        600 
SDAAYGILKE PLTIIHPLLG CSDPYALTRV LHEVEPRYVV LYDAELTFVR QLEIYRASRP 

       610        620        630        640        650        660 
GKPLRVYFLI YGGSTEEQRY LTALRKEKEA FEKLIREKAS MVVPEEREGR DETNLDLARG 

       670        680        690        700        710        720 
SAALDAPTDT RKAGGQEQNG TQSSIVVDMR EFRSELPSLI HRRGIDIEPV TLEVGDYILT 

       730        740        750        760        770        780 
PELCVERKSV SDLIGSLHSG RLYSQCLAMS RYYRRPVLLI EFDPSKPFSL APRGAFFQEM 

       790        800        810        820        830        840 
SSSDVSSKLT LLTLHFPRLR LLWCPSPHAT AELFEELKQN KPQPDAATAM AITADSETLP 

       850        860        870        880        890        900 
ESDRYNPGPQ DFVLKMPGVN AKNCRSLMNQ VKNIAELATL SLERLTTILG HSGNAKQLHD 

       910 
FLHTAYADLV SKGRVRK 

References and documentation are available.

Number of amino acids: 917

Molecular weight: 103689.07

Theoretical pI: 7.97

Amino acid composition: 
Ala (A)  66	  7.2%
Arg (R)  69	  7.5%
Asn (N)  30	  3.3%
Asp (D)  43	  4.7%
Cys (C)  18	  2.0%
Gln (Q)  31	  3.4%
Glu (E)  80	  8.7%
Gly (G)  47	  5.1%
His (H)  21	  2.3%
Ile (I)  38	  4.1%
Leu (L) 119	 13.0%
Lys (K)  57	  6.2%
Met (M)  13	  1.4%
Phe (F)  33	  3.6%
Pro (P)  47	  5.1%
Ser (S)  63	  6.9%
Thr (T)  50	  5.5%
Trp (W)   6	  0.7%
Tyr (Y)  27	  2.9%
Val (V)  59	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 123
Total number of positively charged residues (Arg + Lys): 126

Atomic composition:

Carbon      C	      4607
Hydrogen    H	      7394
Nitrogen    N	      1290
Oxygen      O	      1365
Sulfur      S	        31

Formula: C4607H7394N1290O1365S31
Total number of atoms: 14687

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    74355
Abs 0.1% (=1 g/l)   0.717, assuming all pairs of Cys residues form cystines


Ext. coefficient    73230
Abs 0.1% (=1 g/l)   0.706, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.39
This classifies the protein as unstable.



Aliphatic index: 92.63

Grand average of hydropathicity (GRAVY): -0.340