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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPHEEQIWK SKLLKAANND MDMDRNVPLA PNLNVNMNMK MNASRNGDEF GLTSSRFDGV 

        70         80         90        100        110        120 
VIGSNGDVNF KPILEKIFRE LTSDYKEERK LASISLFDLL VSLEHELSIE EFQAVSNDIN 

       130        140        150        160        170        180 
NKILELVHTK KTSTRVGAVL SIDTLISFYA YTERLPNETS RLAGYLRGLI PSNDVEVMRL 

       190        200        210        220        230        240 
AAKTLGKLAV PGGTYTSDFV EFEIKSCLEW LTASTEKNSF SSSKPDHAKH AALLIITALA 

       250        260        270        280        290        300 
ENCPYLLYQY LNSILDNIWR ALRDPHLVIR IDASITLAKC LSTLRNRDPQ LTSQWVQRLA 

       310        320        330        340        350        360 
TSCEYGFQVN TLECIHASLL VYKEILFLKD PFLNQVFDQM CLNCIAYENH KAKMIREKIY 

       370        380        390        400        410        420 
QIVPLLASFN PQLFAGKYLH QIMDNYLEIL TNAPANKIPH LKDDKPQILI SIGDIAYEVG 

       430        440        450        460        470        480 
PDIAPYVKQI LDYIEHDLQT KFKFRKKFEN EIFYCIGRLA VPLGPVLGKL LNRNILDLMF 

       490        500        510        520        530        540 
KCPLSDYMQE TFQILTERIP SLGPKINDEL LNLVCSTLSG TPFIQPGSPM EIPSFSRERA 

       550        560        570        580        590        600 
REWRNKNILQ KTGESNDDNN DIKIIIQAFR MLKNIKSRFS LVEFVRIVAL SYIEHTDPRV 

       610        620        630        640        650        660 
RKLAALTSCE IYVKDNICKQ TSLHSLNTVS EVLSKLLAIT IADPLQDIRL EVLKNLNPCF 

       670        680        690        700        710        720 
DPQLAQPDNL RLLFTALHDE SFNIQSVAME LVGRLSSVNP AYVIPSIRKI LLELLTKLKF 

       730        740        750        760        770        780 
STSSREKEET ASLLCTLIRS SKDVAKPYIE PLLNVLLPKF QDTSSTVAST ALRTIGELSV 

       790        800        810        820        830        840 
VGGEDMKIYL KDLFPLIIKT FQDQSNSFKR EAALKALGQL AASSGYVIDP LLDYPELLGI 

       850        860        870        880        890        900 
LVNILKTENS QNIRRQTVTL IGILGAIDPY RQKEREVTST TDISTEQNAP PIDIALLMQG 

       910        920        930        940        950        960 
MSPSNDEYYT TVVIHCLLKI LKDPSLSSYH TAVIQAIMHI FQTLGLKCVS FLDQIIPTIL 

       970        980        990       1000       1010       1020 
DVMRTCSQSL LEFYFQQLCS LIIIVRQHIR PHVDSIFQAI KDFSSVAKLQ ITLVSVIEAI 

      1030       1040       1050       1060       1070       1080 
SKALEGEFKR LVPLTLTLFL VILENDKSSD KVLSRRVLRL LESFGPNLEG YSHLITPKIV 

      1090       1100       1110       1120       1130       1140 
QMAEFTSGNL QRSAIITIGK LAKDVDLFEM SSRIVHSLLR VLSSTTSDEL SKVIMNTLSL 

      1150       1160       1170       1180       1190       1200 
LLIQMGTSFA IFIPVINEVL MKKHIQHTIY DDLTNRILNN DVLPTKILEA NTTDYKPAEQ 

      1210       1220       1230       1240       1250       1260 
MEAADAGVAK LPINQSVLKS AWNSSQQRTK EDWQEWSKRL SIQLLKESPS HALRACSNLA 

      1270       1280       1290       1300       1310       1320 
SMYYPLAKEL FNTAFACVWT ELYSQYQEDL IGSLCIALSS PLNPPEIHQT LLNLVEFMEH 

      1330       1340       1350       1360       1370       1380 
DDKALPIPTQ SLGEYAERCH AYAKALHYKE IKFIKEPENS TIESLISINN QLNQTDAAIG 

      1390       1400       1410       1420       1430       1440 
ILKHAQQHHS LQLKETWFEK LERWEDALHA YNEREKAGDT SVSVTLGKMR SLHALGEWEQ 

      1450       1460       1470       1480       1490       1500 
LSQLAARKWK VSKLQTKKLI APLAAGAAWG LGEWDMLEQY ISVMKPKSPD KEFFDAILYL 

      1510       1520       1530       1540       1550       1560 
HKNDYDNASK HILNARDLLV TEISALINES YNRAYSVIVR TQIITEFEEI IKYKQLPPNS 

      1570       1580       1590       1600       1610       1620 
EKKLHYQNLW TKRLLGCQKN VDLWQRVLRV RSLVIKPKQD LQIWIKFANL CRKSGRMRLA 

      1630       1640       1650       1660       1670       1680 
NKALNMLLEG GNDPSLPNTF KAPPPVVYAQ LKYIWATGAY KEALNHLIGF TSRLAHDLGL 

      1690       1700       1710       1720       1730       1740 
DPNNMIAQSV KLSSASTAPY VEEYTKLLAR CFLKQGEWRI ATQPNWRNTN PDAILGSYLL 

      1750       1760       1770       1780       1790       1800 
ATHFDKNWYK AWHNWALANF EVISMVQEET KLNGGKNDDD DDTAVNNDNV RIDGSILGSG 

      1810       1820       1830       1840       1850       1860 
SLTINGNRYP LELIQRHVVP AIKGFFHSIS LLETSCLQDT LRLLTLLFNF GGIKEVSQAM 

      1870       1880       1890       1900       1910       1920 
YEGFNLMKIE NWLEVLPQLI SRIHQPDPTV SNSLLSLLSD LGKAHPQALV YPLTVAIKSE 

      1930       1940       1950       1960       1970       1980 
SVSRQKAALS IIEKIRIHSP VLVNQAELVS HELIRVAVLW HELWYEGLED ASRQFFVEHN 

      1990       2000       2010       2020       2030       2040 
IEKMFSTLEP LHKHLGNEPQ TLSEVSFQKS FGRDLNDAYE WLNNYKKSKD INNLNQAWDI 

      2050       2060       2070       2080       2090       2100 
YYNVFRKITR QIPQLQTLDL QHVSPQLLAT HDLELAVPGT YFPGKPTIRI AKFEPLFSVI 

      2110       2120       2130       2140       2150       2160 
SSKQRPRKFS IKGSDGKDYK YVLKGHEDIR QDSLVMQLFG LVNTLLKNDS ECFKRHLDIQ 

      2170       2180       2190       2200       2210       2220 
QYPAIPLSPK SGLLGWVPNS DTFHVLIREH RDAKKIPLNI EHWVMLQMAP DYENLTLLQK 

      2230       2240       2250       2260       2270       2280 
IEVFTYALDN TKGQDLYKIL WLKSRSSETW LERRTTYTRS LAVMSMTGYI LGLGDRHPSN 

      2290       2300       2310       2320       2330       2340 
LMLDRITGKV IHIDFGDCFE AAILREKYPE KVPFRLTRML TYAMEVSGIE GSFRITCENV 

      2350       2360       2370       2380       2390       2400 
MRVLRDNKES LMAILEAFAL DPLIHWGFDL PPQKLTEQTG IPLPLINPSE LLRKGAITVE 

      2410       2420       2430       2440       2450       2460 
EAANMEAEQQ NETKNARAML VLRRITDKLT GNDIKRFNEL DVPEQVDKLI QQATSIERLC 

      2470 
QHYIGWCPFW


References and documentation are available. 

Number of amino acids: 2470

Molecular weight: 281139.79

Theoretical pI: 6.81


Amino acid composition: 
Ala (A) 156	  6.3%
Arg (R) 112	  4.5%
Asn (N) 132	  5.3%
Asp (D) 124	  5.0%
Cys (C)  31	  1.3%
Gln (Q) 111	  4.5%
Glu (E) 160	  6.5%
Gly (G)  93	  3.8%
His (H)  61	  2.5%
Ile (I) 192	  7.8%
Leu (L) 330	 13.4%
Lys (K) 164	  6.6%
Met (M)  50	  2.0%
Phe (F)  89	  3.6%
Pro (P) 112	  4.5%
Ser (S) 186	  7.5%
Thr (T) 128	  5.2%
Trp (W)  36	  1.5%
Tyr (Y)  76	  3.1%
Val (V) 127	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%



Total number of negatively charged residues (Asp + Glu): 284
Total number of positively charged residues (Arg + Lys): 276

Atomic composition:

Carbon      C	     12676
Hydrogen    H	     20165
Nitrogen    N	      3371
Oxygen      O	      3672
Sulfur      S	        81

Formula: C12676H20165N3371O3672S81
Total number of atoms: 39965

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   313115
Abs 0.1% (=1 g/l)   1.114, assuming all pairs of Cys residues form cystines


Ext. coefficient   311240
Abs 0.1% (=1 g/l)   1.107, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.00
This classifies the protein as unstable.



Aliphatic index: 103.65

Grand average of hydropathicity (GRAVY): -0.168