ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPIIALLIG NGGREHTIAW KLCESPLISK VYVAPGNGGT ASNGAESKME NVNIGVCDFE 

        70         80         90        100        110        120 
QLVKFALDKD VNLVIPGPEL PLVEGIEGHF RRVGIPCFGP SALAARMEGS KVFSKDFMHR 

       130        140        150        160        170        180 
NNIPTAVYKS FSNYDHAKSF LDTCTFDVVI KADGLAAGKG VIIPKTKKEA FEALESIMLN 

       190        200        210        220        230        240 
EEFGSAGKNV VIEELLEGEE LSILTFSDGY TCRSLPPAQD HKRAFDGDKG PNTGGMGCYA 

       250        260        270        280        290        300 
PTPVASPKLL ETVQSTIIQP TIDGMRHEGY PLVGILFTGL MLTPSGPRVL EYNVRFGDPE 

       310        320        330        340        350        360 
TQAVLPLLES DLAEIILACV NHRLDAIDIV ISRKFSCAVV CVAGGYPGPY NKGDIITFDA 

       370        380        390        400        410        420 
LKDKNTRIFH AGTSIRDGNV VTNGGRVLAV EATGDSVEAA VRLAYEGVKT VHFDKMFYRK 

       430        440        450        460        470        480 
DIAHHALNPK RKTREILTYE NSGVSVDNGN EFVQRIKDLV KSTRRPGADA DIGGFGGIFD 

       490        500        510        520        530        540 
LKQAGWNDPL LVSATDGVGS KLLIALSLNK HDTVGIDLVA MNVNDLVVQG AEPLIFLDYF 

       550        560        570        580        590        600 
ATGSLDLKVS TSFVEGVVKG CKQAGCALVG GETSEMPGLY HDGHYDANGT SVGAVSRDDI 

       610        620        630        640        650        660 
LPKPESFSKG DILLGLASDG VHSNGYSLVR KIVEYSDLEY TSVCPWDKNV RLGDSLLIPT 

       670        680        690        700        710        720 
RIYVKPLLHV IRKNIVKGMA HITGGGLVEN VPRMLPSHLN AIIDVDTWEV PEVFKWLKDA 

       730        740        750        760        770        780 
GNVPISDMAR TFNMGIGMVV AVASEDAEET MKELTSVGET VYRIGQLVDK ESSSERCHLV 


NLNKWETF 

References and documentation are available.

Number of amino acids: 788

Molecular weight: 85231.47

Theoretical pI: 5.37

Amino acid composition: 
Ala (A)  56	  7.1%
Arg (R)  31	  3.9%
Asn (N)  36	  4.6%
Asp (D)  51	  6.5%
Cys (C)  13	  1.6%
Gln (Q)  10	  1.3%
Glu (E)  50	  6.3%
Gly (G)  78	  9.9%
His (H)  19	  2.4%
Ile (I)  50	  6.3%
Leu (L)  72	  9.1%
Lys (K)  47	  6.0%
Met (M)  17	  2.2%
Phe (F)  30	  3.8%
Pro (P)  38	  4.8%
Ser (S)  50	  6.3%
Thr (T)  40	  5.1%
Trp (W)   6	  0.8%
Tyr (Y)  20	  2.5%
Val (V)  74	  9.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 101
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      3796
Hydrogen    H	      6018
Nitrogen    N	      1018
Oxygen      O	      1147
Sulfur      S	        30

Formula: C3796H6018N1018O1147S30
Total number of atoms: 12009

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63550
Abs 0.1% (=1 g/l)   0.746, assuming all pairs of Cys residues form cystines


Ext. coefficient    62800
Abs 0.1% (=1 g/l)   0.737, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.28
This classifies the protein as stable.



Aliphatic index: 94.72

Grand average of hydropathicity (GRAVY): -0.039