ProtParam
User-provided sequence:
10 20 30 40 50 60
MEPLISAPYL TTTKMSAPAT LDAACIFCKI IKSEIPSFKL IETKYSYAFL DIQPTAEGHA
70 80 90 100 110 120
LIIPKYHGAK LHDIPDEFLT DAMPIAKRLA KAMKLDTYNV LQNNGKIAHQ EVDHVHFHLI
130 140 150
PKRDEKSGLI VGWPAQETDF DKLGKLHKEL LAKLEGSD
References and
documentation are available.
Number of amino acids: 158
Molecular weight: 17679.56
Theoretical pI: 6.44
Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 19
Atomic composition:
Carbon C 805
Hydrogen H 1273
Nitrogen N 205
Oxygen O 229
Sulfur S 6
Formula: C
805H
1273N
205O
229S
6
Total number of atoms: 2518
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 13075
Abs 0.1% (=1 g/l) 0.740, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.732, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.66
This classifies the protein as stable.
Aliphatic index: 96.46
Grand average of hydropathicity (GRAVY): -0.194