ProtParam
User-provided sequence:
10 20 30 40 50 60
MEPPKGDTVV KTEVPVKDPS LSVVDSKTKG VEDANTEIAL SDEVEIETKG SDSTPVKAPS
70 80 90 100 110 120
RTSSGSKKSV HWSPELVSGS QEPDQKAASS SSAGSNPYIA RSPAETSDAS LKDTMETVKG
130 140 150 160 170 180
VLGRWGKRVA EAAKKTESLA GNTWQHLRTA PSFADAAMGR IAQSTKVFAE GGYEKIFRQT
190 200 210 220 230 240
FETDPEEQLL NSFACYLSTS AGPVMGVLYI SSAKLAYCSD NPLSYKNGDQ TEWSYYKVVI
250 260 270 280 290
PLHQLKAVNP SASIVNPAEK YIQVISVDNH EFWFMGFLNY DGAVTSLQDS LQAGALRSV
References and
documentation are available.
Number of amino acids: 299
Molecular weight: 32214.95
Theoretical pI: 5.27
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 1418
Hydrogen H 2225
Nitrogen N 379
Oxygen O 463
Sulfur S 7
Formula: C
1418H
2225N
379O
463S
7
Total number of atoms: 4492
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 42525
Abs 0.1% (=1 g/l) 1.320, assuming all pairs of Cys residues form cystines
Ext. coefficient 42400
Abs 0.1% (=1 g/l) 1.316, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.58
This classifies the protein as unstable.
Aliphatic index: 71.77
Grand average of hydropathicity (GRAVY): -0.412