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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPQEEFITT EEVEQEIVPT VEVEQDVPVD IEGENDDDDE MMNDDEEALE VDMSNNSLTY

70 80 90 100 110 120
FDKHTDSVFA IGHHPNLPLV CTGGGDNLAH LWTSHSQPPK FAGTLTGYGE SVISCSFTSE

130 140 150 160 170 180
GGFLVTADMS GKVLVHMGQK GGAQWKLASQ MQEVEEIVWL KTHPTIARTF AFGATDGSVW

190 200 210 220 230 240
CYQINEQDGS LEQLMSGFVH QQDCSMGEFI NTDKGENTLE LVTCSLDSTI VAWNCFTGQQ

250 260 270 280 290 300
LFKITQAEIK GLEAPWISLS LAPETLTKGN SGVVACGSNN GLLAVINCNN GGAILHLSTV

310 320 330 340 350 360
IELKPEQDEL DASIESISWS SKFSLMAIGL VCGEILLYDT SAWRVRHKFV LEDSVTKLMF

370 380 390 400 410 420
DNDDLFASCI NGKVYQFNAR TGQEKFVCVG HNMGVLDFIL LHPVANTGTE QKRKVITAGD

430
EGVSLVFEVP N


References and documentation are available.

Number of amino acids: 431 Molecular weight: 47166.90 Theoretical pI: 4.35
Amino acid composition: 
Ala (A) 24 5.6% Arg (R) 5 1.2% Asn (N) 22 5.1% Asp (D) 27 6.3% Cys (C) 11 2.6% Gln (Q) 20 4.6% Glu (E) 38 8.8% Gly (G) 37 8.6% His (H) 12 2.8% Ile (I) 23 5.3% Leu (L) 38 8.8% Lys (K) 18 4.2% Met (M) 12 2.8% Phe (F) 20 4.6% Pro (P) 13 3.0% Ser (S) 32 7.4% Thr (T) 30 7.0% Trp (W) 8 1.9% Tyr (Y) 5 1.2% Val (V) 36 8.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 65 Total number of positively charged residues (Arg + Lys): 23 Atomic composition: Carbon C 2075 Hydrogen H 3210 Nitrogen N 538 Oxygen O 671 Sulfur S 23 Formula: C2075H3210N538O671S23 Total number of atoms: 6517 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 52075 Abs 0.1% (=1 g/l) 1.104, assuming all pairs of Cys residues form cystines Ext. coefficient 51450 Abs 0.1% (=1 g/l) 1.091, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 36.96 This classifies the protein as stable. Aliphatic index: 84.99 Grand average of hydropathicity (GRAVY): -0.123