ProtParam
User-provided sequence:
10 20 30 40 50 60
MEPQEEFITT EEVEQEIVPT VEVEQDVPVD IEGENDDDDE MMNDDEEALE VDMSNNSLTY
70 80 90 100 110 120
FDKHTDSVFA IGHHPNLPLV CTGGGDNLAH LWTSHSQPPK FAGTLTGYGE SVISCSFTSE
130 140 150 160 170 180
GGFLVTADMS GKVLVHMGQK GGAQWKLASQ MQEVEEIVWL KTHPTIARTF AFGATDGSVW
190 200 210 220 230 240
CYQINEQDGS LEQLMSGFVH QQDCSMGEFI NTDKGENTLE LVTCSLDSTI VAWNCFTGQQ
250 260 270 280 290 300
LFKITQAEIK GLEAPWISLS LAPETLTKGN SGVVACGSNN GLLAVINCNN GGAILHLSTV
310 320 330 340 350 360
IELKPEQDEL DASIESISWS SKFSLMAIGL VCGEILLYDT SAWRVRHKFV LEDSVTKLMF
370 380 390 400 410 420
DNDDLFASCI NGKVYQFNAR TGQEKFVCVG HNMGVLDFIL LHPVANTGTE QKRKVITAGD
430
EGVSLVFEVP N
References and
documentation are available.
Number of amino acids: 431
Molecular weight: 47166.90
Theoretical pI: 4.35
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 2075
Hydrogen H 3210
Nitrogen N 538
Oxygen O 671
Sulfur S 23
Formula: C
2075H
3210N
538O
671S
23
Total number of atoms: 6517
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52075
Abs 0.1% (=1 g/l) 1.104, assuming all pairs of Cys residues form cystines
Ext. coefficient 51450
Abs 0.1% (=1 g/l) 1.091, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.96
This classifies the protein as stable.
Aliphatic index: 84.99
Grand average of hydropathicity (GRAVY): -0.123