ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPSKQEVPK LMETPPNISN DSSATEKGEA TRQQQLPNNR YALTVDEVIE QHIGALGFAQ 

        70         80         90        100        110        120 
ILHALLVSIA WIFDAQTTLI SIFSDAQPAA RLLATGAIVE GASLCGLASG EWEWIGPKSD 

       130        140        150        160        170        180 
TVVSEWNLIC QHKFLVAVPS TLFFIGSLFG SGVYGYLADS WFGRKKTLLL SCVLTFVTAF 

       190        200        210        220        230        240 
AISFSPNVWV YAFLRFANGF FRSGIGSCCI VLATEIVGKK WRGQVGQYGF FFFTLGFLSL 

       250        260        270        280        290        300 
PLMAYLERKS WRNLYRIISF LPLGYAVCLL PFAYESPRWL LVKGRNKEAM VVLKKLARLN 

       310        320        330        340        350        360 
GKQLPADLSL VDPIPERDDQ TSSSEKFWKT KWAVKRIIMV MMAGFGSGFV YYGIQLNAEN 

       370        380        390        400        410        420 
LNFNLYLTVA VNALMEFPAV FIGSFLLGVM NRRPLFSNSS YLAGFACLLC AVLSIHRVIR 

       430        440        450        460        470        480 
AISVAKWLQL AVEAVGFMAS STAYDVLYVY CVELFPTNVR NTAVSLLRQA FMLGASAAPL 

       490        500        510        520        530 
LVALGRESAM MSFIVFGVAS VLSGIVSLWL RETRNAPLYE TLAQQGKAEE IENETIMIT 

References and documentation are available.

Number of amino acids: 539

Molecular weight: 59659.75

Theoretical pI: 8.72

Amino acid composition: 
Ala (A)  54	 10.0%
Arg (R)  24	  4.5%
Asn (N)  21	  3.9%
Asp (D)  11	  2.0%
Cys (C)   9	  1.7%
Gln (Q)  18	  3.3%
Glu (E)  28	  5.2%
Gly (G)  37	  6.9%
His (H)   4	  0.7%
Ile (I)  31	  5.8%
Leu (L)  68	 12.6%
Lys (K)  21	  3.9%
Met (M)  14	  2.6%
Phe (F)  36	  6.7%
Pro (P)  21	  3.9%
Ser (S)  44	  8.2%
Thr (T)  24	  4.5%
Trp (W)  13	  2.4%
Tyr (Y)  17	  3.2%
Val (V)  44	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      2752
Hydrogen    H	      4270
Nitrogen    N	       692
Oxygen      O	       742
Sulfur      S	        23

Formula: C2752H4270N692O742S23
Total number of atoms: 8479

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    97330
Abs 0.1% (=1 g/l)   1.631, assuming all pairs of Cys residues form cystines


Ext. coefficient    96830
Abs 0.1% (=1 g/l)   1.623, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.43
This classifies the protein as stable.



Aliphatic index: 105.32

Grand average of hydropathicity (GRAVY): 0.408