ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPTFDIPDS TDWLDTPLTL LAPFETSLRC QVCKDFFDNP VITSCSHTFC SLCIRRCLST 

        70         80         90        100        110        120 
EGKCPTCRSS DQELKLRRNW VVQELVEGFK NARPSILQLA RMAQTGTDDS GDLAAEEPAS 

       130        140        150        160        170        180 
KKRKIEPNAI VGTDGLPEEG IRTRSQSRGA SRQPQATPVQ VIDDGNDEDY MPDGLVPCPV 

       190        200        210        220        230        240 
CGRRMKEEAV FRHLDSCTGT AEELKPAAFG AYRSLAPGPR KSFLAATGKP PERLPVINYS 

       250        260        270        280        290        300 
LLKDTVLRKK LKDLGIPNWG PRALLQRRHT EWLNLWNANC DSRTPKPKRE LLRELDVWER 

       310        320        330        340        350        360 
TQGGNSVTPT DPTNAVMNKD FNTEEWSANY DTDFKALIAN ARKKNDAVIR STIPNASQAN 

       370        380        390        400        410        420 
SDTPRSAQLV DQPIEASLTP QDVDEKSTMN PQDAIDNRTE VPPVPDPPQA LSGIDRAVNS 

       430        440        450 
PMKNRDRHSD SSELQQWLGL RRPLSTSVFN 

References and documentation are available.

Number of amino acids: 450

Molecular weight: 50148.40

Theoretical pI: 5.82

Amino acid composition: 
Ala (A)  33	  7.3%
Arg (R)  36	  8.0%
Asn (N)  24	  5.3%
Asp (D)  36	  8.0%
Cys (C)  12	  2.7%
Gln (Q)  19	  4.2%
Glu (E)  27	  6.0%
Gly (G)  21	  4.7%
His (H)   4	  0.9%
Ile (I)  16	  3.6%
Leu (L)  40	  8.9%
Lys (K)  23	  5.1%
Met (M)   7	  1.6%
Phe (F)  12	  2.7%
Pro (P)  39	  8.7%
Ser (S)  34	  7.6%
Thr (T)  32	  7.1%
Trp (W)   8	  1.8%
Tyr (Y)   4	  0.9%
Val (V)  23	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2168
Hydrogen    H	      3468
Nitrogen    N	       640
Oxygen      O	       690
Sulfur      S	        19

Formula: C2168H3468N640O690S19
Total number of atoms: 6985

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50710
Abs 0.1% (=1 g/l)   1.011, assuming all pairs of Cys residues form cystines


Ext. coefficient    49960
Abs 0.1% (=1 g/l)   0.996, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.45
This classifies the protein as unstable.



Aliphatic index: 70.69

Grand average of hydropathicity (GRAVY): -0.692