ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEPTFDIPDS TDWLDTPLTL LAPFETSLRC QVCKDFFDNP VITSCSHTFC SLCIRRCLST 

        70         80         90        100        110        120 
EGKCPTCRSS DQELKLRRNW VVQELVEGFK NARPSILQLA RMAQTGTDDS GDLAAEEPAS 

       130        140        150        160        170        180 
KKRKIEPNAI VGTDGLPEEG IRTRSQSRGA SRQPQATPVQ VIDDGNDEDY MPDGLVPCPV 

       190        200        210        220        230        240 
CGRRMKEEAV FRHLDSCTGT AEELKPAAFG SLAPGPRKSF LAATGKPPER LPVINYSLLK 

       250        260        270        280        290        300 
DTVLRKKLKD LGIPNWGPRA LLQRRHTEWL NLWNANCDSR TPKPKRELLR ELDVWERTQG 

       310        320        330        340        350        360 
GNSVTPTDPT NAVMNKDFNT EEWSANYDTD FKALIANARK KNDAVIRSTI PNASQANSDT 

       370        380        390        400        410        420 
PRSAQLVDQP IEASLTPQDV DEKSTMNPQD AIDNRTEVPP VPDPPQALSG IDRAVNSPMK 

       430        440 
NVTEGDAQAI PISSSASTHK TPH 

References and documentation are available.

Number of amino acids: 443

Molecular weight: 48906.98

Theoretical pI: 5.51

Amino acid composition: 
Ala (A)  35	  7.9%
Arg (R)  31	  7.0%
Asn (N)  23	  5.2%
Asp (D)  35	  7.9%
Cys (C)  12	  2.7%
Gln (Q)  18	  4.1%
Glu (E)  27	  6.1%
Gly (G)  21	  4.7%
His (H)   5	  1.1%
Ile (I)  18	  4.1%
Leu (L)  36	  8.1%
Lys (K)  24	  5.4%
Met (M)   7	  1.6%
Phe (F)  11	  2.5%
Pro (P)  40	  9.0%
Ser (S)  33	  7.4%
Thr (T)  34	  7.7%
Trp (W)   7	  1.6%
Tyr (Y)   3	  0.7%
Val (V)  23	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2112
Hydrogen    H	      3384
Nitrogen    N	       618
Oxygen      O	       679
Sulfur      S	        19

Formula: C2112H3384N618O679S19
Total number of atoms: 6812

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43720
Abs 0.1% (=1 g/l)   0.894, assuming all pairs of Cys residues form cystines


Ext. coefficient    42970
Abs 0.1% (=1 g/l)   0.879, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.17
This classifies the protein as unstable.



Aliphatic index: 70.50

Grand average of hydropathicity (GRAVY): -0.657