ProtParam
User-provided sequence:
10 20 30 40 50 60
MEQAVRSESK KGQVTGTDLI NEVSKAKKCT VIGGSGFLGQ HMVEQLLSRG YAVNVFDVRQ
70 80 90 100 110 120
GFDNPRVQFF IGDLCNQQDL YPALKGVSTV FHCASPPSNS NNKELFYRVN STGTKTVIET
130 140 150 160 170 180
CKEAGVQKLI LTSSASVVFE GVDIKNGTED LPYAMKPIDY YTETKILQER AVLDANDPKK
190 200 210 220 230 240
NFLTAAIRPH GIFGPRDPQL VPVLIDAARK GKMKFMIGNG KNLVDFTFVE NVVHGHILAA
250 260 270 280 290 300
EHLSRDAGLG GKAFHITNDE PIPFWTFLSR ILTGLNYEAP KYHIPYRVAY YLAFLLSLLV
310 320 330 340 350 360
MVLSPLIQIQ TTFTPFRVAL AGTFHYYSCE KAKKLIGYRP LVTMDDAVER TVQSFHHLRK
DK
References and
documentation are available.
Number of amino acids: 362
Molecular weight: 40411.61
Theoretical pI: 8.99
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1830
Hydrogen H 2871
Nitrogen N 489
Oxygen O 519
Sulfur S 12
Formula: C
1830H
2871N
489O
519S
12
Total number of atoms: 5721
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26610
Abs 0.1% (=1 g/l) 0.658, assuming all pairs of Cys residues form cystines
Ext. coefficient 26360
Abs 0.1% (=1 g/l) 0.652, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.02
This classifies the protein as stable.
Aliphatic index: 89.92
Grand average of hydropathicity (GRAVY): -0.129