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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEQFLKGLPK VELHAHLNGS LGIKSLCDLG ERLYGTSCKD FLKLCAHFSR FEKDMDACFE

70 80 90 100 110 120
KFAFVHELTS TREGLRFATE LAIRDFAEDN VQYVEMRTTP KANENYSRRD YLQIVIDAIK

130 140 150 160 170 180
AASETYPEIT VKLLPSINRA EPVDVAEETV SLAVELARAH PNLILGIDLS GNPGKGRFSD

190 200 210 220 230 240
FAPILAQARD KGLKLAIHCA EIENPSEVKE MLHFGMSRCG HGTFLTPEDI GQLKQRNIAI

250 260 270 280 290 300
ECCLTSNVKS GTVPSLEEHH LKRIMEADAP KVICTDDSGV FDTTLTKEFL IAAETFGLTR

310 320 330
EQCIDLTLEA VHHSFASEQE QIQMADRVGN YADILVK


References and documentation are available.

Number of amino acids: 337 Molecular weight: 37575.90 Theoretical pI: 5.30
Amino acid composition: 
Ala (A) 30 8.9% Arg (R) 17 5.0% Asn (N) 11 3.3% Asp (D) 20 5.9% Cys (C) 10 3.0% Gln (Q) 10 3.0% Glu (E) 32 9.5% Gly (G) 19 5.6% His (H) 12 3.6% Ile (I) 21 6.2% Leu (L) 36 10.7% Lys (K) 20 5.9% Met (M) 7 2.1% Phe (F) 17 5.0% Pro (P) 12 3.6% Ser (S) 19 5.6% Thr (T) 20 5.9% Trp (W) 0 0.0% Tyr (Y) 6 1.8% Val (V) 18 5.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 52 Total number of positively charged residues (Arg + Lys): 37 Atomic composition: Carbon C 1657 Hydrogen H 2635 Nitrogen N 453 Oxygen O 508 Sulfur S 17 Formula: C1657H2635N453O508S17 Total number of atoms: 5270 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 9565 Abs 0.1% (=1 g/l) 0.255, assuming all pairs of Cys residues form cystines Ext. coefficient 8940 Abs 0.1% (=1 g/l) 0.238, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 35.75 This classifies the protein as stable. Aliphatic index: 90.36 Grand average of hydropathicity (GRAVY): -0.194