ProtParam
User-provided sequence:
10 20 30 40 50 60
MEQFLKGLPK VELHAHLNGS LGIKSLCDLG ERLYGTSCKD FLKLCAHFSR FEKDMDACFE
70 80 90 100 110 120
KFAFVHELTS TREGLRFATE LAIRDFAEDN VQYVEMRTTP KANENYSRRD YLQIVIDAIK
130 140 150 160 170 180
AASETYPEIT VKLLPSINRA EPVDVAEETV SLAVELARAH PNLILGIDLS GNPGKGRFSD
190 200 210 220 230 240
FAPILAQARD KGLKLAIHCA EIENPSEVKE MLHFGMSRCG HGTFLTPEDI GQLKQRNIAI
250 260 270 280 290 300
ECCLTSNVKS GTVPSLEEHH LKRIMEADAP KVICTDDSGV FDTTLTKEFL IAAETFGLTR
310 320 330
EQCIDLTLEA VHHSFASEQE QIQMADRVGN YADILVK
References and
documentation are available.
Number of amino acids: 337
Molecular weight: 37575.90
Theoretical pI: 5.30
Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1657
Hydrogen H 2635
Nitrogen N 453
Oxygen O 508
Sulfur S 17
Formula: C
1657H
2635N
453O
508S
17
Total number of atoms: 5270
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 9565
Abs 0.1% (=1 g/l) 0.255, assuming all pairs of Cys residues form cystines
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.238, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.75
This classifies the protein as stable.
Aliphatic index: 90.36
Grand average of hydropathicity (GRAVY): -0.194