ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEQMHSLESS LPPEQPPTKQ AIESLNLELS QEFKLAANAV TRLYRVANEK NSLTKHQGYL 

        70         80         90        100        110        120 
TCLDDILCAL DSNVTADELR AWCYKRRNDI LSNSQDKSLN PVKERERKLN KFSENQHREN 

       130        140        150        160        170        180 
EAHKEPFEKD SAVKYNFSFN ESNGDLSNIN ENIAPKFRLS MPPLSVEHPP RNASRIKSWK 

       190        200        210        220        230 
ARTINHGRGD TRNLNDITGL GHERERDREN THYEKKPKLD SDSEVDIRSF RQDMDL 

References and documentation are available.

Number of amino acids: 236

Molecular weight: 27361.40

Theoretical pI: 6.47

Amino acid composition: 
Ala (A)  13	  5.5%
Arg (R)  19	  8.1%
Asn (N)  22	  9.3%
Asp (D)  16	  6.8%
Cys (C)   3	  1.3%
Gln (Q)   8	  3.4%
Glu (E)  23	  9.7%
Gly (G)   6	  2.5%
His (H)   8	  3.4%
Ile (I)   9	  3.8%
Leu (L)  23	  9.7%
Lys (K)  18	  7.6%
Met (M)   4	  1.7%
Phe (F)   7	  3.0%
Pro (P)  12	  5.1%
Ser (S)  22	  9.3%
Thr (T)   9	  3.8%
Trp (W)   2	  0.8%
Tyr (Y)   5	  2.1%
Val (V)   7	  3.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1176
Hydrogen    H	      1873
Nitrogen    N	       359
Oxygen      O	       381
Sulfur      S	         7

Formula: C1176H1873N359O381S7
Total number of atoms: 3796

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18575
Abs 0.1% (=1 g/l)   0.679, assuming all pairs of Cys residues form cystines


Ext. coefficient    18450
Abs 0.1% (=1 g/l)   0.674, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.19
This classifies the protein as unstable.



Aliphatic index: 66.99

Grand average of hydropathicity (GRAVY): -1.107