ProtParam
User-provided sequence:
10 20 30 40 50 60
MERLQRLMMN SKVGSADTGR DDTKETVYIS SIALLKMLKH GRAGVPMEVM GLMLGEFVDD
70 80 90 100 110 120
YTVNVVDVFA MPQSGTGVSV EAVDDVFQAK MMDMLKQTGR DQMVVGWYHS HPGFGCWLSS
130 140 150 160 170 180
VDVNTQKSFE QLNSRAVAVV VDPIQSVKGK VVIDAFRLID TGALINNLEP RQTTSNTGLL
190 200 210 220 230 240
NKANIQALIH GLNRHYYSLN IDYHKTAKET KMLMNLHKEQ WQSGLKMYDY EEKEESNLAA
250 260 270 280 290 300
TKSMVKIAEQ YSKRIEEEKE LTEEELKTRY VGRQDPKKHL SETADETLEN NIVSVLTAGV
NSVAIK
References and
documentation are available.
Number of amino acids: 306
Molecular weight: 34398.29
Theoretical pI: 5.81
Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1504
Hydrogen H 2421
Nitrogen N 415
Oxygen O 471
Sulfur S 17
Formula: C
1504H
2421N
415O
471S
17
Total number of atoms: 4828
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31400
Abs 0.1% (=1 g/l) 0.913, assuming all pairs of Cys residues form cystines
Ext. coefficient 31400
Abs 0.1% (=1 g/l) 0.913, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.04
This classifies the protein as stable.
Aliphatic index: 85.62
Grand average of hydropathicity (GRAVY): -0.383