ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MERPFGCFFI LLLISYTVVA TFDDEPSFPE NADLTKDLEQ KCFSINKVDP NLKFENDRLK 

        70         80         90        100        110        120 
RAYIALQAWK KAIYSDPFKT TANWVGSDVC SYNGVYCAPA LDDDSLTVVA GVDLNHADIA 

       130        140        150        160        170        180 
GHLPPELGLM TDLALFHINS NRFCGIIPKS LSKLALMYEF DVSNNRFVGQ FPEVSLSWPS 

       190        200        210        220        230        240 
LKFLDLRYNE FEGSLPSEIF DKDLDAIFLN NNRFESVIPG TIGKSKASVV TFANNKFSGC 

       250        260        270        280        290        300 
IPKSIGNMKN LNEIVFTGNN LTGCFPNEIG LLNNVTVFDA SKNGFVGSLP STLSGLASVE 

       310        320        330        340        350        360 
QLDLSHNKLT GFVVDKFCKL PNLDSFKFSY NFFNGEAESC VPGRNNGKQF DDTNNCLQNR 

       370        380        390        400        410        420 
PSQKPAKQCL PVVSRPVDCS KDKCSGGSNG GSSPSPNPPR TSEPKPSKPE PVMPKPSDSS 

       430        440        450        460        470        480 
KPETPKTPEQ PSPKPQPPKH ESPKPEEPEN KHELPKQKES PKPQPSKPED SPKPEQPKPE 

       490        500        510        520        530        540 
ESPKPEQPQI PEPTKPVSPP NEAQGPTPDD PYDASPVKNR RSPPPPKVED TRVPPPQPPM 

       550        560        570        580        590        600 
PSPSPPSPIY SPPPPVHSPP PPVYSSPPPP HVYSPPPPVA SPPPPSPPPP VHSPPPPPVF 

       610        620        630        640        650        660 
SPPPPVFSPP PPSPVYSPPP PSHSPPPPVY SPPPPTFSPP PTHNTNQPPM GAPTPTQAPT 

       670        680        690        700        710        720 
PSSETTQVPT PSSESDQSQI LSPVQAPTPV QSSTPSSEPT QVPTPSSSES YQAPNLSPVQ 

       730        740        750        760        770        780 
APTPVQAPTT SSETSQVPTP SSESNQSPSQ APTPILEPVH APTPNSKPVQ SPTPSSEPVS 

       790        800        810        820        830        840 
SPEQSEEVEA PEPTPVNPSS VPSSSPSTDT SIPPPENNDD DDDGDFVLPP HIGFQYASPP 


PPMFQGY 

References and documentation are available.

Number of amino acids: 847

Molecular weight: 90989.58

Theoretical pI: 4.93

Amino acid composition: 
Ala (A)  34	  4.0%
Arg (R)  14	  1.7%
Asn (N)  48	  5.7%
Asp (D)  41	  4.8%
Cys (C)  13	  1.5%
Gln (Q)  35	  4.1%
Glu (E)  49	  5.8%
Gly (G)  32	  3.8%
His (H)  13	  1.5%
Ile (I)  23	  2.7%
Leu (L)  45	  5.3%
Lys (K)  47	  5.5%
Met (M)   8	  0.9%
Phe (F)  37	  4.4%
Pro (P) 179	 21.1%
Ser (S) 110	 13.0%
Thr (T)  45	  5.3%
Trp (W)   3	  0.4%
Tyr (Y)  17	  2.0%
Val (V)  54	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 90
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      4067
Hydrogen    H	      6212
Nitrogen    N	      1048
Oxygen      O	      1283
Sulfur      S	        21

Formula: C4067H6212N1048O1283S21
Total number of atoms: 12631

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42580
Abs 0.1% (=1 g/l)   0.468, assuming all pairs of Cys residues form cystines


Ext. coefficient    41830
Abs 0.1% (=1 g/l)   0.460, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 83.24
This classifies the protein as unstable.



Aliphatic index: 53.81

Grand average of hydropathicity (GRAVY): -0.736