ProtParam
User-provided sequence:
10 20 30 40 50 60
MERYDRQLRL WGALGQDSLN RSRVCVVGPA TPLLQEVFKN LVLAGISSLT WLKVECAVQS
70 80 90 100 110 120
GSLFLAELKK DLEPLASKQL EYEENDLRKT LQQPQYDWTR FSVVILTCIG EQTAMLDLNE
130 140 150 160 170 180
IRRQRGTKFP PVLNTFVSGF YGYIYLVLSE THFVLQAHPD SKKYDLRLQN PWPELINYVD
190 200 210 220 230 240
TFDLSKMDTA TFSGIPYTVL LMKCIAKLER DGNNGRITID QMKKVLDQIC LPLGNDVIYE
250 260 270 280 290 300
PNYVEAKRYA YLACSQNDCC KELEDLLRNL EISDYGNDWH DTYNYEILTL LLTLKNIAKE
310 320 330 340 350 360
NGELSFQPLT GTLPDMESTT ENYIRLKKLY EVKAKLDKSR VEESLARSKK IVSQDVLETF
370 380 390 400 410 420
CSHYGEVRKI LPPKSDLLGI FSTSNALLDA LVMVQFWEQP AVTAEDKDEF IGLRVDDNYS
430 440 450 460
VMAFFGGAVV QEAIKLITHH YVPIDNLFLY NGINNSSATY KI
References and
documentation are available.
Number of amino acids: 462
Molecular weight: 52852.61
Theoretical pI: 5.20
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 2382
Hydrogen H 3740
Nitrogen N 614
Oxygen O 708
Sulfur S 17
Formula: C
2382H
3740N
614O
708S
17
Total number of atoms: 7461
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 67770
Abs 0.1% (=1 g/l) 1.282, assuming all pairs of Cys residues form cystines
Ext. coefficient 67270
Abs 0.1% (=1 g/l) 1.273, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.85
This classifies the protein as stable.
Aliphatic index: 98.94
Grand average of hydropathicity (GRAVY): -0.217